| Content Provider | Springer Nature : BioMed Central |
|---|---|
| Author | Ellis, Danielle R Sors, Thomas G Brunk, Dennis G Albrecht, Carrie Orser, Cindy Lahner, Brett Wood, Karl V Harris, Hugh H Pickering, Ingrid J Salt, David E |
| Abstract | Background It has become increasingly evident that dietary Se plays a significant role in reducing the incidence of lung, colorectal and prostate cancer in humans. Different forms of Se vary in their chemopreventative efficacy, with Se-methylselenocysteine being one of the most potent. Interestingly, the Se accumulating plant Astragalus bisulcatus (Two-grooved poison vetch) contains up to 0.6% of its shoot dry weight as Se-methylselenocysteine. The ability of this Se accumulator to biosynthesize Se-methylselenocysteine provides a critical metabolic shunt that prevents selenocysteine and selenomethionine from entering the protein biosynthetic machinery. Such a metabolic shunt has been proposed to be vital for Se tolerance in A. bisulcatus. Utilization of this mechanism in other plants may provide a possible avenue for the genetic engineering of Se tolerance in plants ideally suited for the phytoremediation of Se contaminated land. Here, we describe the overexpression of a selenocysteine methyltransferase from A. bisulcatus to engineer Se-methylselenocysteine metabolism in the Se non-accumulator Arabidopsis thaliana (Thale cress). Results By over producing the A. bisulcatus enzyme selenocysteine methyltransferase in A. thaliana, we have introduced a novel biosynthetic ability that allows the non-accumulator to accumulate Se-methylselenocysteine and γ-glutamylmethylselenocysteine in shoots. The biosynthesis of Se-methylselenocysteine in A. thaliana also confers significantly increased selenite tolerance and foliar Se accumulation. Conclusion These results demonstrate the feasibility of developing transgenic plant-based production of Se-methylselenocysteine, as well as bioengineering selenite resistance in plants. Selenite resistance is the first step in engineering plants that are resistant to selenate, the predominant form of Se in the environment. |
| Related Links | https://bmcplantbiol.biomedcentral.com/counter/pdf/10.1186/1471-2229-4-1.pdf |
| Ending Page | 11 |
| Page Count | 11 |
| Starting Page | 1 |
| File Format | HTM / HTML |
| ISSN | 14712229 |
| DOI | 10.1186/1471-2229-4-1 |
| Journal | BMC Plant Biology |
| Issue Number | 1 |
| Volume Number | 4 |
| Language | English |
| Publisher | BioMed Central |
| Publisher Date | 2004-01-28 |
| Access Restriction | Open |
| Subject Keyword | Plant Sciences Agriculture Tree Biology Selenite SeMet Selenocysteine HFBA Selenate Reduction |
| Content Type | Text |
| Resource Type | Article |
| Subject | Plant Science |
| Journal Impact Factor | 4.3/2023 |
| 5-Year Journal Impact Factor | 5.2/2023 |
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