NDLI: Molecular cloning and characterization of the mouse Acdp gene family

Content Provider	Springer Nature : BioMed Central
Author	Wang, Cong-Yi Yang, Ping Shi, Jing-Da Purohit, Sharad Guo, Dehuang An, Haiqian Gu, Jian-Guo Ling, Jennifer Dong, Zheng She, Jin-Xiong
Abstract	Background We have recently cloned and characterized a novel gene family named ancient conserved domain protein (ACDP) in humans. To facilitate the functional study of this novel gene family, we have cloned and characterized Acdp, the mouse homologue of the human ACDP gene family. Results The four Acdp genes (Acdp1, Acdp2, Acdp3 and Acdp4) contain 3,631 bp, 3,244 bp, 2,684 bp and 2,743 bp of cDNA sequences, and encode deduced proteins of 951, 874, 713 and 771 amino acids, respectively. The mouse Acdp genes showed very strong homologies (>90%) in both nucleotide and amino acid sequences to their human counterparts. In addition, both nucleotide and amino acid sequences within the Ancient Conserved Domain (ACD) are highly conserved in many different taxonomic species. Particularly, Acdp proteins showed very strong AA homologies to the bacteria CorC protein (35% AA identity with 55% homology), which is involved in magnesium and cobalt efflux. The Acdp genes are widely expressed in all tissues tested except for Acdp1, which is only highly expressed in the brain with low levels of expression in kidney and testis. Immunostaining of Acdp1 in hippocampus neurons revealed a predominant localization on the plasma membrane. Conclusion The Acdp genes are evolutionarily conserved in diverse species and ubiquitously expressed throughout development and adult tissues suggesting that Acdp may be an essential gene. Acdp showed strong homology to bacteria CorC protein and predominantly localized on the plasma membrane. These results suggest that Acdp is probably a family of proteins involved in ion transport in mammalian cells
Related Links	https://bmcgenomics.biomedcentral.com/counter/pdf/10.1186/1471-2164-5-7.pdf
Ending Page	9
Page Count	9
Starting Page	1
File Format	HTM / HTML
ISSN	14712164
DOI	10.1186/1471-2164-5-7
Journal	BMC Genomics
Issue Number	1
Volume Number	5
Language	English
Publisher	BioMed Central
Publisher Date	2004-01-15
Access Restriction	Open
Subject Keyword	Life Sciences Microarrays Proteomics Animal Genetics and Genomics Microbial Genetics and Genomics Plant Genetics and Genomics Radiation Hybrid Amino Acid Homology DUF21 Domain Brain Tissue Extract High Amino Acid Homology
Content Type	Text
Resource Type	Article
Subject	Biotechnology Genetics
Journal Impact Factor	3.5/2023
5-Year Journal Impact Factor	4.1/2023

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Cloning, characterization and expression analysis of porcine microRNAs

Characterization of the mouse Dazap1 gene encoding an RNA-binding protein that interacts with infertility factors DAZ and DAZL

Characterization and gene expression analysis of the cir multi-gene family of plasmodium chabaudi chabaudi (AS)

In silico characterization of the family of PARP-like poly(ADP-ribosyl)transferases (pARTs)

A comprehensive characterization of the caspase gene family in insects from the order Lepidoptera

Evolutionary patterns of amino acid substitutions in 12 Drosophila genomes

Gene duplication in an African cichlid adaptive radiation

Brain transcriptional stability upon prion protein-encoding gene invalidation in zygotic or adult mouse

The actin multigene family of Paramecium tetraurelia

Molecular cloning and characterization of the mouse Acdp gene family

Similar Documents

Cloning, characterization and expression analysis of porcine microRNAs

Characterization of the mouse Dazap1 gene encoding an RNA-binding protein that interacts with infertility factors DAZ and DAZL

Characterization and gene expression analysis of the cir multi-gene family of plasmodium chabaudi chabaudi (AS)

In silico characterization of the family of PARP-like poly(ADP-ribosyl)transferases (pARTs)

A comprehensive characterization of the caspase gene family in insects from the order Lepidoptera

Evolutionary patterns of amino acid substitutions in 12 Drosophila genomes

Gene duplication in an African cichlid adaptive radiation

Brain transcriptional stability upon prion protein-encoding gene invalidation in zygotic or adult mouse

The actin multigene family of Paramecium tetraurelia

Molecular cloning and characterization of the mouse Acdp gene family