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Purification and characterization of chitinase from Alcaligenes faecalis AU02 by utilizing marine wastes and its antioxidant activity
| Content Provider | Springer Nature : BioMed Central |
|---|---|
| Author | Annamalai, Neelamegam Veeramuthu Rajeswari, Mayavan Vijayalakshmi, Shanmugam Balasubramanian, Thangavel |
| Abstract | Marine waste is an abundant renewable source for the recovery of several value added metabolites with potential industrial applications. This study describes the production of chitinase on marine waste, with the subsequent use of the same marine waste for the extraction of antioxidants. A chitinase-producing bacterium isolated from seafood effluent was identified as Alcaligenes faecalis AU02. Optimal chitinase production was obtained in culture conditions of 37°C for 72 h in 100 ml medium containing 1% shrimp and crab shell powder (1:1) (w/v), 0.1% K2HPO4, and 0.05% MgSO4·7H2O. The molecular weight of chitinase was determined by SDS-PAGE to be 36 kDa. The optimum pH, temperature, pH stability, and thermal stability of chitinase were about 8, 37°C, 5–12, and 40–80°C, respectively. The antioxidant activity of A. faecalis AU02 culture supernatant was determined through scavenging ability on 1,1-diphenyl-2-picrylhydrazyl (DPPH) as 84%, and the antioxidant compound was characterized by TLC and its FT-IR spectrum. The present study proposed that marine wastes can be utilized to generate a high-value-added product and that pharmacological studies can extend its use to the field of medicine. |
| Related Links | https://annalsmicrobiology.biomedcentral.com/counter/pdf/10.1007/s13213-011-0198-5.pdf |
| Ending Page | 807 |
| Page Count | 7 |
| Starting Page | 801 |
| File Format | HTM / HTML |
| ISSN | 18692044 |
| DOI | 10.1007/s13213-011-0198-5 |
| Journal | Annals of Microbiology |
| Issue Number | 4 |
| Volume Number | 61 |
| Language | English |
| Publisher | BioMed Central |
| Publisher Date | 2011-01-22 |
| Access Restriction | Open |
| Subject Keyword | Microbiology Microbial Genetics and Genomics Microbial Ecology Mycology Applied Microbiology Alcaligenes faecalis Chitinase Marine waste Antioxidant |
| Content Type | Text |
| Resource Type | Article |
| Subject | Applied Microbiology and Biotechnology |
| Journal Impact Factor | 3/2023 |
| 5-Year Journal Impact Factor | 3.4/2023 |
