| Content Provider | Springer Nature : BioMed Central |
|---|---|
| Author | Okada, Shigeru List, Edward O. Sankaran, Sudha Kopchick, John J. |
| Abstract | Introduction Early detection, assessment of disease progression, and application of an appropriate therapeutic intervention are all important for the care of patients with type 2 diabetes. Currently, however, there is no simple test for early detection of type 2 diabetes. Established diagnostic tests for the disease including oral glucose tolerance, fasting blood glucose, and hemoglobin A1c are relatively late markers where the disease has already progressed. Since blood is in direct contact with many tissues, we hypothesized that pathological tissue changes are likely to be reflected in proteomic profiles of plasma. Methods Mice were reared either on regular chow or a high-fat diet at weaning and several physiological responses (i.e., weight, fasting plasma glucose and insulin, and glucose tolerance) were monitored at regular time intervals. Plasma was collected at regular intervals for proteomic analysis by two-dimensional gel electrophoresis and subsequent mass spectrometry. Results Onset of hyperinsulinemia with corresponding glucose intolerance was observed in 2 weeks and fasting blood glucose levels rose significantly after 4 weeks on the high-fat diet. Many proteins were found to exist in multiple forms (isoforms). Levels of some isoforms including plasma retinol binding protein, transthyretin, Apolipoprotein A1, and kininogen showed significant changes as early as 4 weeks which coincided with the very early development of glucose intolerance. Conclusions These results show that a proteomic approach to study the development of type 2 diabetes may uncover unknown early post-translationally modified diagnostic and/or therapeutic protein targets. |
| Related Links | https://clinicalproteomicsjournal.biomedcentral.com/counter/pdf/10.1007/s12014-009-9040-5.pdf |
| Ending Page | 17 |
| Page Count | 12 |
| Starting Page | 6 |
| File Format | HTM / HTML |
| ISSN | 15590275 |
| DOI | 10.1007/s12014-009-9040-5 |
| Journal | Clinical Proteomics |
| Issue Number | 1 |
| Volume Number | 6 |
| Language | English |
| Publisher | BioMed Central |
| Publisher Date | 2010-02-06 |
| Access Restriction | Open |
| Subject Keyword | Cell Biology Pathology Biotechnology Proteomics Diabetes Biomarkers Mice Protein isoforms Two-dimensional gel electrophoresis Plasma |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biotechnology Biochemistry (medical) |
| Journal Impact Factor | 2.8/2023 |
| 5-Year Journal Impact Factor | 3.9/2023 |
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