NDLI: In vitro propagation and ex vitro rooting of Aerva lanata (L.) Juss. ex Schult.: a rare medicinal plant

Content Provider	Springer Nature Link
Author	Revathi, J. Shekhawat, Mahipal S. Manokari, M.
Copyright Year	2016
Abstract	The present study describes an improved and reproducible in vitro regeneration system for Aerva lanata using nodal segment explants obtained from a mature 2 years old plant. Direct organogenesis of shoots from nodal segments was achieved by culturing on Murashige and Skoog (MS) medium supplemented with 1.5 mg l$^{−1}$ 6-benzylaminopurine (BAP). On this media combination, 8.7 ± 0.21 shoots per explant were induced. Shoots multiplication was extensively influenced by the type and the concentrations of plant growth regulators used, repeated transfer of mother explants and the sub-culturing of in vitro regenerated shoot clumps on fresh medium. Maximum numbers of shoots (49.39 ± 1.24) was observed on MS medium augmented with 1.0 mg l$^{−1}$ BAP and 0.2 mg l$^{−1}$ α-naphthalene acetic acid. Full, half and one-fourth strength of MS medium with different concentrations of auxins was tested for in vitro root formation. One fourth strength MS medium with 2.0 mg l$^{−1}$ indole-3 butyric acid (IBA) was optimum for in vitro root induction (36.9 ± 0.72 roots per shoot). Experiments on ex vitro rooting were conducted to shorten the regeneration pathway and observed highest percentage (100 %) of rooting from the cut ends of the shoots with 200 mg l$^{−1}$ IBA. About 12.6 ± 0.30 roots were induced from the shoots using this method. The well rooted plantlets were acclimatized successfully in Soilrite under the greenhouse conditions with 92 % survival rate.
Starting Page	40
Ending Page	47
Page Count	8
File Format	PDF
ISSN	00195502
Journal	Indian Journal of Plant Physiology
Volume Number	22
Issue Number	1
e-ISSN	09740252
Language	English
Publisher	Springer India
Publisher Date	2016-10-08
Publisher Place	New Delhi
Access Restriction	Subscribed
Subject Keyword	Hardening Regeneration Plant Biochemistry Conservation Aerva lanata Plant Physiology Plant Sciences Ex vitro rooting Plant Ecology Cell Biology Plant Genetics & Genomics
Content Type	Text
Resource Type	Article
Subject	Plant Science Agronomy and Crop Science

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