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| Content Provider | Springer Nature Link |
|---|---|
| Author | Leifeng, Zhao Fuzhao, Nian |
| Copyright Year | 2014 |
| Abstract | The complete mRNA sequence of one tobacco (Nicotiana tabacum) gene-leucine aminopeptidase was amplified through rapid amplification of cDNA ends (RACE) method. The full-length mRNA was 2,051 bp containing an 1,584-bp open reading frame, which encodes a protein of 527 amino acids. Sequence analysis revealed that the deduced tobacco leucine aminopeptidase protein shares high homology with the leucine aminopeptidase of wine grape (84 %), populus trichocarpa (79 %), thale cress (77 %), and triticum urartu (74 %). Results also showed that tobacco leucine aminopeptidase gene has a close genetic relationship with the leucine aminopeptidase gene of wine grape. The expression profile was studied and result indicated that tobacco leucine aminopeptidase gene was differentially expressed in detected tobacco tissues including leaf, stem, root, and flower. Our experiment established the foundation for further research on this tobacco gene. |
| Starting Page | 201 |
| Ending Page | 205 |
| Page Count | 5 |
| File Format | |
| ISSN | 01008404 |
| Journal | Brazilian Journal of Botany |
| Volume Number | 37 |
| Issue Number | 2 |
| e-ISSN | 18069959 |
| Language | English |
| Publisher | Springer International Publishing |
| Publisher Date | 2014-05-04 |
| Publisher Place | Cham |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Tobacco Leucine aminopeptidase Expression profile Plant Systematics/Taxonomy/Biogeography RACE Sequence analysis |
| Content Type | Text |
| Resource Type | Article |
| Subject | Plant Science |
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