NDLI: Generation of HSC-Like Cells from Human Embryonic Stem Cells by Inhibition of TGF-βR2 Signaling

Please wait, while we are loading the content...

Molecular and Probiotic Functional Characterization of Lactobacillus spp. Associated with Traditionally Fermented Fish, Tungtap of Meghalaya in Northeast India

Emergence of Aggressive Population in the Bipolaris sorokiniana of Barley (Hordeum vulgare L.) Through Anastomosis

Abundance and Spatio-Temporal Distributional Pattern of Dotilla Myctiroides (Milne-Edwards) on Exposed Sandy Beach of Aksa, Mumbai

Scanning Electron Microscopy and Molecular Characterization of Aedes albopictus (Skuse)

Functional Screening and Molecular Characterization of Halophilic and Halotolerant Bacteria by 16S rRNA Gene Sequence Analysis

Functional Characterization of Bubaline Recombinant-Cathelicidin

Molecular Characterization of Commercial Varieties of Phyllanthus emblica Using RAPD and Nuclear rDNA SNPs

Evaluation of Probiotic Potential of New Bacterial Strain, Lactobacillus spicheri G2 Isolated from Gundruk

Effect of Preconceptional Exposure of Triazophos Formulation on Fertility and Reproductive Performance of Female Wistar Rats, Rattus norvegicus

Characterisation and Validation of House Keeping Gene for Expression Analysis in Catla catla (Hamilton)

Transferability of SSR Markers of Brassica sp. to Some Popular Varieties of Brassica Juncea

Development of Novel Microsatellite Markers in Jatropha curcas and Evaluation of Their Cross-Species Transferability

Generation of HSC-Like Cells from Human Embryonic Stem Cells by Inhibition of TGF-βR2 Signaling

Analysis of Genetic Diversity in 21 Genotypes of Indian Banana Using RAPDs and IRAPs Markers

Chloroplast and Mitochondrial DNA Assay in Solving Issues Related to the Taxonomy of Beveragial Tea Clones

Effects of Phenanthrene and Pyrene on Cytogenetic Stability of Human Dermal Fibroblasts Using Alkaline Comet Assay Technique

In-Silico Designing of Molecular Beacon Probes for Sensitive and Rapid Detection of Water Borne Pathogenic Bacteria

Bactericidal Activity of Nanopolymers Against Shrimp Pathogenic Bacterium Vibrio harveyi

Biochemical Characterization of a RIL Population of Rice (Oryza sativa L.) Under Direct-Seeded Aerobic and Transplanted Conditions

Bioprospecting and molecular characterization of laccase producing bacteria from paper mills of Himachal Pradesh

Guanine and Cytosine at the Second Codon Position Influence Gene Expression in Cereals

Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 85, Issue 3, September 2015

Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 85, Issue 2, June 2015

Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 85, Issue 1, March 2015

Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 84

Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 83

Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 82

Generation of HSC-Like Cells from Human Embryonic Stem Cells by Inhibition of TGF-βR2 Signaling

Content Provider	Springer Nature Link
Author	Kazemi Sheykhshabani, Sedigheh Hashemzadeh Chaleshtori, Morteza Khosravi Farsani, Somayeh Ghasemi Dehkordi, Payam Ganji Arjenaki, Mahboubeh Abdian, Narges Allahbakhshian Farsani, Mehdi
Copyright Year	2015
Abstract	The efficient induction of embryonic stem cells into hematopoietic stem cells (HSCs) requires understanding the pro or inhibitory regulatory networks, which are involved in these pathways. Transforming growth factor β family is a super family of growth factors such as TGF-β1, TGF-β2, TGF-β3, and some other cytokines. Previous works indicate that some of TGF-β subfamilies have negative regulatory effect during hematopoietic differentiation and HSC proliferation. The inhibitory effects of TGF-β1 in early stage of differentiation of endothelial and hematopoietic cells have been shown. Therefore, in the present study, in order to evaluate the promotion of hematopoietic differentiation, the activities of TGF-β regulatory factor were reduced through knockdown of TGF-βR2 expression. The ESCs were transduced by Lentivirus carrying SiTGF-βR2 and its scramble separately. Green fluorescent protein (GFP) was used as a marker to monitor gene expression. Six days post transduction GFP$^{+}$ colonies were used for EB generation and the next day, the same size EBs were transferred in differentiation media involved in defining cytokines. The expression level of hematopoietic-specific genes was evaluated in 0, 7, and 15-days of differentiation. The real-time PCR analysis in 15 day EBs showed 2–3-folds increase in SiTGF-βR2 as compared with the scramble. Therefore, this finding may be confirmed if TGF receptor silencing can reduce the inhibitory effect of TGF-β during early hematopoietic differentiation.
Starting Page	1017
Ending Page	1026
Page Count	10
File Format	PDF
ISSN	03698211
Journal	Proceedings of the National Academy of Sciences, India Section B: Biological Sciences
Volume Number	85
Issue Number	4
e-ISSN	22501746
Language	English
Publisher	Springer India
Publisher Date	2015-04-08
Publisher Place	New Delhi
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Life Sciences Plant Biochemistry Nucleic Acid Chemistry TGF-βR2 signaling Behavioral Sciences HSCs ESCs
Content Type	Text
Resource Type	Article
Subject	Environmental Science Agricultural and Biological Sciences

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

The Generation of Definitive Endoderm from Human Embryonic Stem Cells is Initially Independent from Activin A but Requires Canonical Wnt-Signaling

Identification of Differentially Expressed Human Endogenous Retrovirus Families in Human Leukemia and Lymphoma Cell Lines and Stem Cells

Generation of Mesenchymal Stem Cells from Human Embryonic Stem Cells in a Complete Serum-free Condition

Scalable Generation of Mesenchymal Stem Cells from Human Embryonic Stem Cells in 3D

Simultaneous Suppression of TGF-β and ERK Signaling Contributes to the Highly Efficient and Reproducible Generation of Mouse Embryonic Stem Cells from Previously Considered Refractory and Non-permissive Strains

Stagewise keratinocyte differentiation from human embryonic stem cells by defined signal transduction modulators

Retinoic Acid Induces Embryonic Stem Cells (ESCs) Transition to 2 Cell-Like State Through a Coordinated Expression of Dux and Duxbl1

Promotion of In Vitro Hair Cell-like Cell Differentiation from Human Embryonic Stem Cells through the Regulation of Notch Signaling

Robust generation of hepatocyte-like cells from human embryonic stem cell populations.

Generation of HSC-Like Cells from Human Embryonic Stem Cells by Inhibition of TGF-βR2 Signaling

Similar Documents

The Generation of Definitive Endoderm from Human Embryonic Stem Cells is Initially Independent from Activin A but Requires Canonical Wnt-Signaling

Identification of Differentially Expressed Human Endogenous Retrovirus Families in Human Leukemia and Lymphoma Cell Lines and Stem Cells

Generation of Mesenchymal Stem Cells from Human Embryonic Stem Cells in a Complete Serum-free Condition

Scalable Generation of Mesenchymal Stem Cells from Human Embryonic Stem Cells in 3D

Simultaneous Suppression of TGF-β and ERK Signaling Contributes to the Highly Efficient and Reproducible Generation of Mouse Embryonic Stem Cells from Previously Considered Refractory and Non-permissive Strains

Stagewise keratinocyte differentiation from human embryonic stem cells by defined signal transduction modulators

Retinoic Acid Induces Embryonic Stem Cells (ESCs) Transition to 2 Cell-Like State Through a Coordinated Expression of Dux and Duxbl1

Promotion of In Vitro Hair Cell-like Cell Differentiation from Human Embryonic Stem Cells through the Regulation of Notch Signaling

Robust generation of hepatocyte-like cells from human embryonic stem cell populations.

Generation of HSC-Like Cells from Human Embryonic Stem Cells by Inhibition of TGF-βR2 Signaling