NDLI: Proteomic Analysis of Diversified Extremophilic Strains of Pseudomonas in the Presence of Cadmium

Content Provider	Springer Nature Link
Author	Bhatt, Arun Jain, Sourabh
Copyright Year	2013
Abstract	Environmental contamination caused by heavy metals, such as cadmium (Cd), is a serious and growing concern. Cd is a highly toxic and carcinogenic metal for humans, animals and plants and is a widespread pollutant with a long biological life. The presence and persistence of growth promoting metal-resistant microbial community in the soil system facilitates remediation of Cd. This study deals with proteomic characterization of cadmium-resistant diversified isolates of Pseudomonas spp., i.e., psychrotolerant P. putida (isolate SB32) and alkalophilic P. monteilli (isolate SB35). Further, two-dimensional gel electrophoresis (2DE) revealed 24 proteins in SB32 and 21 proteins in SB35, differentially expressed after Cd exposure. On the basis of their increased expression, two protein spots in SB32 and one in SB35 were selected for further analysis. With MS/MS spectra produced by LC–MS analysis, spots were identified through NCBI EST database searching with Mascot MS/MS Ion Search Engines (Matrix Sciences). Spots from cadmium-treated P. putida SB32 appear to be ATP-dependent Clp protease subunit and 20S proteasome, A and B subunits, while spot from cadmium-treated P. monteilli SB35 appears to be nitroreductase. Protein identification supports that interplay between protein folding and hydrolysis may be a central mechanism for proper cell function survival in the presence of cadmium.
Starting Page	354
Ending Page	359
Page Count	6
File Format	PDF
ISSN	2249720X
Journal	Agricultural Research
Volume Number	2
Issue Number	4
e-ISSN	22497218
Language	English
Publisher	Springer India
Publisher Date	2013-10-15
Publisher Place	India
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cadmium Plant Biochemistry Proteomics Psychrotolerant Alkalophilic Pseudomonas Biodiversity Plant Sciences Cell Biology Plant Ecology Plant Genetics & Genomics
Content Type	Text
Resource Type	Article
Subject	Plant Science Food Science Agronomy and Crop Science

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