NDLI: A Rapid and Low Cost Method of DNA Isolation for Marker Assisted Selection in Rice

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A Rapid and Low Cost Method of DNA Isolation for Marker Assisted Selection in Rice

Content Provider	Springer Nature Link
Author	Kurrey, L. N. Saxena, R. R. Xalxo, S. M. Pali, V. Verulkar, S. B.
Copyright Year	2014
Abstract	A simple, rapid, and efficient method of leaf DNA extraction was optimized for PCR based Marker Assisted Selection (MAS) in rice. The procedure involved homogenization of leaves of 10-days old seedlings in the extraction buffer, followed by centrifugation. The supernatant was directly used for PCR. The isolated DNA had protein and RNA impurities. However, these impurities did not affect PCR amplification. Results of comparative analysis of PCR products using the DNA extracted by this method with that using standard CTAB method clearly revealed that the intensity, sharpness, and pattern of PCR amplification were similar. The low cost and time saving method reported here is highly suitable for large scale MAS in rice.
Starting Page	83
Ending Page	86
Page Count	4
File Format	PDF
ISSN	2249720X
Journal	Agricultural Research
Volume Number	3
Issue Number	1
e-ISSN	22497218
Language	English
Publisher	Springer India
Publisher Date	2014-02-22
Publisher Place	India
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	DNA extraction Plant Biochemistry Biodiversity Plant Sciences MAS Rice Cell Biology Plant Ecology Plant Genetics & Genomics
Content Type	Text
Resource Type	Article
Subject	Plant Science Food Science Agronomy and Crop Science

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