NDLI: Simple Maturation of Direct-Converted Hepatocytes Derived from Fibroblasts

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Tissue Engineering and Regenerative Medicine : Volume 14, Issue 4, August 2017

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Simple Maturation of Direct-Converted Hepatocytes Derived from Fibroblasts

Content Provider	Springer Nature Link
Author	Kim, Yohan Seo, Daekwan Yoon, Sangtae Cho, Young duck Lee, Seung Bum Jeong, Jaemin Choi, Dongho Ryu, Kiyoung Kang, Kyojin
Copyright Year	2017
Abstract	Target cells differentiation techniques from stem cells are developed rapidly. Recently, direct conversion techniques are introduced in various categories. Unlike pluripotent stem cells, this technique enables direct differentiation into the other cell types such as neurons, cardiomyocytes, insulin-producing cells, and hepatocytes without going through the pluripotent stage. However, the function of these converted cells reserve an immature phenotype. Therefore, we modified the culture conditions of mouse direct converted hepatocytes (miHeps) to mature fetal characteristics, such as higher AFP and lower albumin (ALB) expression than primary hepatocytes. First, we generate miHeps from mouse embryonic fibroblasts (MEFs) with two transcription factors HNF4α and Foxa3. These cells indicate typical epithelial morphology and express hepatic proteins. To mature hepatic function, DMSO is treated during culture time for more than 7 days. After maturation, miHeps showed features of maturation such as exhibiting typical hepatocyte-like morphology, increased up-regulated ALB and CYP enzyme gene expression, down-regulated AFP expressions, and acquired hepatic function over time. Thus, our data provides a simple method to mature direct converted hepatocytes functionally and these cells enable them to move closer to generating functional hepatocytes.
Starting Page	579
Ending Page	586
Page Count	8
File Format	PDF
ISSN	17382696
Journal	Tissue Engineering and Regenerative Medicine
Volume Number	14
Issue Number	5
e-ISSN	22125469
Language	English
Publisher	Springer Singapore
Publisher Date	2017-06-23
Publisher Place	Singapore
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Biomedical Engineering Stem Cells Mouse induced hepatocytes Maturation Regenerative Medicine/Tissue Engineering Dimethyl sulfoxide Direct conversion Cell Biology
Content Type	Text
Resource Type	Article
Subject	Medicine Biomedical Engineering

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