NDLI: Phytoestrogen (Daidzein) Promotes Chondrogenic Phenotype of Human Chondrocytes in 2D and 3D Culture Systems

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Phytoestrogen (Daidzein) Promotes Chondrogenic Phenotype of Human Chondrocytes in 2D and 3D Culture Systems

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Tissue Engineering and Regenerative Medicine : Volume 14, Issue 1, February 2017

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Phytoestrogen (Daidzein) Promotes Chondrogenic Phenotype of Human Chondrocytes in 2D and 3D Culture Systems

Content Provider	Springer Nature Link
Author	Yusof, Rohana Ramasamy, Thamil Selvee Snigh, Simmrat Mahmod, Suhaeb A. Rothan, Hussin A. Mei Yee, Yong Djordjevic, Ivan
Copyright Year	2017
Abstract	Clinical investigations have shown a significant relationship between osteoarthritis (OA) and estrogens levels in menopausal women. Therefore, treatment with exogenous estrogens has been shown to decrease the risk of OA. However, the effect estrogen has not been clearly demonstrated in the chondrocytes using phytoestrogens, which lack the specific side-effects of estrogens, may provide an alternative therapy. This study was designed to examine the possible effects of phytoestrogen (daidzein) on human chondrocyte phenotype and extracellular matrix formation. Phytoestrogens which lack the specific side-effects of estrogens may provide beneficial effect without causing hormone based side effect. Human chondrocytes cells were cultured in 2D (flask) and 3D (PCL-CA scaffold) systems. Daidzein cytotoxic effect was determined by MTT assay. Chondrocyte cellular content of glycosaminoglycans (GAGs), total collagen and chondrogenic gene expression were determined in both culture systems after treatment with daidzein. Daidzein showed time-dependent and dose-independent effects on chondrocyte bioactivity. The compound at low doses showed significant (p < 0.05) increase in total collagen and GAGs production at similar levels in 2D and 3D culture environment. The mRNA levels of Collagen II and Sox9 were increased significantly (p < 0.01) after the treatment while the upregulation in COMP expression was statistically insignificant (p > 0.05). The expression levels of Fibronectin, Laminin and Integrin β1 were significantly increased especially in 3D culture system. This study was illustrated the potential positive effects of daidzein on maintenance of human chondrocyte phenotype and extracellular matrix formation suggesting an attractive and viable alternative therapy for OA.
Starting Page	103
Ending Page	112
Page Count	10
File Format	PDF
ISSN	17382696
Journal	Tissue Engineering and Regenerative Medicine
Volume Number	14
Issue Number	2
e-ISSN	22125469
Language	English
Publisher	Korean Tissue Engineering and Regenerative Medicine Society
Publisher Date	2017-03-08
Publisher Place	Seoul
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Biomedical Engineering Stem Cells Phytoestrogens Regenerative Medicine/Tissue Engineering Extracellular matrix PCL-CA scaffold Osteoarthritis Human chondrocyte Cell Biology
Content Type	Text
Resource Type	Article
Subject	Medicine Biomedical Engineering

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Phytoestrogen (Daidzein) Promotes Chondrogenic Phenotype of Human Chondrocytes in 2D and 3D Culture Systems.

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Phytoestrogen (Daidzein) Promotes Chondrogenic Phenotype of Human Chondrocytes in 2D and 3D Culture Systems

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Phytoestrogen (Daidzein) Promotes Chondrogenic Phenotype of Human Chondrocytes in 2D and 3D Culture Systems