NDLI: Synergistic effects of extracellular matrix rigidity and immobilized vascular endothelial growth factor on vascular tube formation and cell morphology of human umbilical vein endothelial cells

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Synergistic effects of extracellular matrix rigidity and immobilized vascular endothelial growth factor on vascular tube formation and cell morphology of human umbilical vein endothelial cells

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Tissue Engineering and Regenerative Medicine : Volume 11

Tissue Engineering and Regenerative Medicine : Volume 10

Tissue Engineering and Regenerative Medicine : Volume 9

Synergistic effects of extracellular matrix rigidity and immobilized vascular endothelial growth factor on vascular tube formation and cell morphology of human umbilical vein endothelial cells

Content Provider	Springer Nature Link
Author	La, Wan Geun Yang, Hee Seok
Copyright Year	2015
Abstract	Tube formation by endothelial cells (EC) is a one of the key factors involved in initial revascularization during the tissue regeneration process. Several previous studies suggested that EC tube formation can be regulated by the stiffness of the culture substrate and immobilization of vascular endothelial growth factor (VEGF). However, the roles of substrate stiffness and chemical signals involved in network formation are unclear. In this study, the effects of substrate rigidity and immobilized VEGF concentration on EC tube formation were examined. Human umbilical vein endothelial cells (HUVEC) were cultured on rigidity-controlled polyacrylamide (PAAM) gels coated by L-DOPA (3,4-dihydroxy-L-phenylalanine), or L-DOPA-Hep (heparin). The HUVECs were cultured on various amounts of immobilized VEGF on the L-DOPA-Hep-coated PAAM (5, 10, and 25 ng). We found that HUVECs formed more tube-like structures when they were cultured on the more rigid substrates, from 1.5 kPa to 144.1 kPa compared to the soft substrate. We also compared various amounts of VEGF immobilized on various rigidity substrates to determine if there was an effect on the tube area of HUVEC. Moreover, we determined an appropriate concentration of VEGF immobilized with various rigidity substrates for vascular tube formation. These findings suggest that there is a synergistic effect of substrate rigidity and chemical signals. Thus, it is possible to combine therapies to synergistically promote an in vitro model of revascularization.
Starting Page	20
Ending Page	27
Page Count	8
File Format	PDF
ISSN	17382696
Journal	Tissue Engineering and Regenerative Medicine
Volume Number	12
Issue Number	1
e-ISSN	22125469
Language	English
Publisher	Korean Tissue Engineering and Regenerative Medicine Society
Publisher Date	2014-12-04
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	tube formation Biomedical Engineering VEGF Human umbilical vein endothelial cells (HUVEC) PAAM Matrix rigidity Biomedicine general Cell Biology
Content Type	Text
Resource Type	Article
Subject	Medicine Biomedical Engineering

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