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| Content Provider | Springer Nature Link |
|---|---|
| Author | Simionescu, Dan Simionescu, Agneta Pascal, Richard Cotoi, Ovidiu Albers, Christopher Terezia, Preda Kenr, Allison Harpa, Marius Sierad, Leslie Rierson, Nicholas Harceaga, Lucian Olah, Peter |
| Copyright Year | 2016 |
| Abstract | The goal of this study was to test the hypothesis that stem cells, as a response to valve-specific extracellular matrix “niches” and mechanical stimuli, would differentiate into valvular interstitial cells (VICs). Porcine aortic root scaffolds were prepared by decellularization. After verifying that roots exhibited adequate hemodynamics in vitro, we seeded human adipose-derived stem cells (hADSCs) within the interstitium of the cusps and subjected the valves to in vitro pulsatile bioreactor testing in pulmonary pressures and flow conditions. As controls we incubated cell-seeded valves in a rotator device which allowed fluid to flow through the valves ensuring gas and nutrient exchange without subjecting the cusps to significant stress. After 24 days of conditioning, valves were analyzed for cell phenotype using immunohistochemistry for vimentin, alpha-smooth muscle cell actin (SMA) and prolyl-hydroxylase (PHA). Fresh native valves were used as immunohistochemistry controls. Analysis of bioreactor-conditioned valves showed that almost all seeded cells had died and large islands of cell debris were found within each cusp. Remnants of cells were positive for vimentin. Cell seeded controls, which were only rotated slowly to ensure gas and nutrient exchange, maintained about 50% of cells alive; these cells were positive for vimentin and negative for alpha-SMA and PHA, similar to native VICs. These results highlight for the first time the extreme vulnerability of hADSCs to valve-specific mechanical forces and also suggest that careful, progressive mechanical adaptation to valve-specific forces might encourage stem cell differentiation towards the VIC phenotype. |
| Starting Page | 507 |
| Ending Page | 515 |
| Page Count | 9 |
| File Format | |
| ISSN | 17382696 |
| Journal | Tissue Engineering and Regenerative Medicine |
| Volume Number | 13 |
| Issue Number | 5 |
| e-ISSN | 22125469 |
| Language | English |
| Publisher | Korean Tissue Engineering and Regenerative Medicine Society |
| Publisher Date | 2016-10-20 |
| Publisher Place | Seoul |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Biomedical Engineering Stem Cells Bioreactor Regenerative Medicine/Tissue Engineering Stem cells Scaffolds Heart valves Cell Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Biomedical Engineering |
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