NDLI: Application of polymerase chain reaction with disposable amplicon detection device for identification of regulatory gene introduced into genetically modified maize

Content Provider	Springer Nature Link
Author	Lee, Ki Jong Suh, Seok Cheol Lim, Myung Ho Cho, Yong Gu Kweon, Soon Jong Chung, Chan Mi Shin, Kong Sik Woo, Hee Jong
Copyright Year	2011
Abstract	A novel genetically modified organism (GMO) detection method was developed using a simple and rapid procedure to identify a regulatory gene introduced into genetically modified (GM) maize. After DNA extraction from GM maize flours, fragments of the conserved sequence of the cauliflower masaic virus (CaMV) 35S promoter gene along with a competitive internal control gene were amplified using duplex polymerase chain reaction (PCR). Amplification products were then detected using a disposable detection device. The quantitative detection limit for GM maize 59122 was found to be 1.0% (w/w). Because the combination-method involving PCR technology coupled with a disposable detection device does not require expensive instrumentation or expertise, it will serve as a valuable alternative to immunoassays and traditional PCR-based tests in the detection of GMOs.
Starting Page	860
Ending Page	864
Page Count	5
File Format	PDF
ISSN	17382203
Journal	Journal of the Korean Society for Applied Biological Chemistry
Volume Number	54
Issue Number	6
e-ISSN	2234344X
Language	English
Publisher	Springer-Verlag
Publisher Date	2011-01-01
Publisher Place	New York
Access Restriction	Subscribed
Subject Keyword	detection genetically modified duplex polymerase chain reaction Applied Microbiology Biological Techniques regulatory gene Bioorganic Chemistry
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Biochemistry, Genetics and Molecular Biology

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