NDLI: Optimization of process variables for the synthesis of silver nanoparticles by Pycnoporus sanguineus using statistical experimental design

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Journal of the Korean Society for Applied Biological Chemistry : Volume 55

Journal of the Korean Society for Applied Biological Chemistry : Volume 54

Journal of the Korean Society for Applied Biological Chemistry : Volume 53

Journal of the Korean Society for Applied Biological Chemistry : Volume 52

Optimization of process variables for the synthesis of silver nanoparticles by Pycnoporus sanguineus using statistical experimental design

Content Provider	Springer Nature Link
Author	Don, Mashitah Mat Chan, Yen San
Copyright Year	2013
Abstract	Sequential optimization strategy based on statistical experimental design and one-factor-at-a-time (OFAT) method were employed to optimize the process parameters for the enhancement of silver nanoparticles (AgNPs) production through biological synthesis using Pycnoporus sanguineus. Based on the OFAT method, three significant components influencing the size of AgNPs produced were identified as AgNO$_{3}$ concentration, incubation temperature, and agitation speed. The optimum values of these process parameter for the synthesis of AgNPs were determined using response surface methodology (RSM) based on Box-Behnken design. The validity of the model developed was verified, and the statistical analysis showed that the optimum operating conditions were 0.001 M of AgNO$_{3}$, 38°C, and 200 rpm with the smallest AgNPs produced at 14.86 nm. The disc diffusion method also suggested that AgNPs produced using optimum conditions have higher antimicrobial activity compared to the unoptimized AgNPs. The present study developed a robust operating condition for the production of AgNPs by P. sanguineus, which was 8.6-fold smaller than that obtained from un-optimized conditions.
Starting Page	11
Ending Page	20
Page Count	10
File Format	PDF
ISSN	17382203
Journal	Journal of the Korean Society for Applied Biological Chemistry
Volume Number	56
Issue Number	1
e-ISSN	2234344X
Language	English
Publisher	The Korean Society for Applied Biological Chemistry
Publisher Date	2013-02-28
Publisher Place	Seoul
Access Restriction	Subscribed
Subject Keyword	Box-Behnken silver nanoparticles (AgNPs) optimization Applied Microbiology Pycnoporus sanguineus Biological Techniques Bioorganic Chemistry
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Biochemistry, Genetics and Molecular Biology

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