NDLI: First formulation of a botanical active substance extracted from neem cell culture for controlling the armyworm

Content Provider	Springer Nature Link
Author	Zuleta Castro, Carolina Rios, Diego Hoyos, Rodrigo Orozco Sánchez, Fernando
Copyright Year	2017
Abstract	Neem (Azadirachta indica) produces several of compounds known as limonoids, which have an antifeedant effect on insects. These compounds are extremely sensitive to some environmental factors that cause their degradation. Despite this, they are widely used in many formulations of commercial bioinsecticides. We evaluated the photodegradation of the crude extract from A. indica cell culture and designed the formulation of a botanical active substance for controlling insects. The crude extract was subjected to 368 nm UV light for 24 h, and its degradation was examined. Limonoids present in the crude extract were analyzed via high-performance liquid chromatography (HPLC). The composition of some compounds in the extract decreased by 55% after 214 min and 83% after 1440 min. For the insect bioassay, we prepared six formulations containing ethanolic extracts from A. indica cell culture as the active ingredient. The formulation also contained a photoprotector and two stabilizers, emulsified with water, castor oil, and Tween 80. Formulations were subjected to stability tests, and the relative phase separation was assessed. To evaluate their biological activity, the antifeedant index and the affected leaf area on corn infested with Spodoptera frugiperda were determined using laboratory- and field-scale bioassays. Three formulations showed good stability, and two presented the highest antifeedant indices (98.5 and 99.7%) in laboratory-scale bioassays. They provided the greatest field-level protection (leaf areas affected were 0.6 and 1.9%, respectively). Therefore, the emulsion containing 0.76% p/p ethanolic extract, 0.72% 8-hydroxyquinoline, 1.00% anthraquinone and epichlorohydrin, 0.20% Tween 80, and 50/50 aqueous phase/oil phase was selected as the best formulation for the insect biocontroller. This thus addresses the problem of metabolite degradation in the field. To our knowledge, this is the first effective formulation of a botanical active substance for controlling insects using A. indica cell culture extract.
Starting Page	1
Ending Page	8
Page Count	8
File Format	PDF
ISSN	17740746
Journal	Agronomy for Sustainable Development
Volume Number	37
Issue Number	5
e-ISSN	17730155
Language	English
Publisher	Springer Paris
Publisher Date	2017-08-29
Publisher Institution	Institut National de la Recherche Agronomique (INRA)
Publisher Place	Paris
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Neem Plant cell culture Limonoids Photodegradation Emulsion Botanical active substance Physical stability Affected leaf area Armyworm Agriculture Soil Science & Conservation Sustainable Development
Content Type	Text
Resource Type	Article
Subject	Environmental Engineering Agronomy and Crop Science

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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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