NDLI: In vitro regeneration of Abelmoschus esculentus L. cv. Wufu: Influence of anti-browning additives on phenolic secretion and callus formation frequency in explants

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In vitro regeneration of Abelmoschus esculentus L. cv. Wufu: Influence of anti-browning additives on phenolic secretion and callus formation frequency in explants

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In vitro regeneration of Abelmoschus esculentus L. cv. Wufu: Influence of anti-browning additives on phenolic secretion and callus formation frequency in explants

Content Provider	Springer Nature Link
Author	Irshad, Muhammad He, Bizhu Liu, Shuang Mitra, Sangeeta Debnath, Biswojit Li, Min Rizwan, Hafiz Muhammad Qiu, Dongliang
Copyright Year	2017
Abstract	Okra (Abelmoschus esculentus L.) belongs to the Malvaceae family and is a difficult species to manipulate and regenerate in vitro. In this study, factors that influence secretion of phenolic compounds, callus induction, and subsequent regeneration in okra were optimized. Different concentrations of anti-browning additives, such as activated charcoal (AC) and citric acid (CA) were tested alone or in combination with ascorbic acid (AA) in MS medium to evaluate their ability to control phenolic secretion from hypocotyl and cotyledon explants. Among the anti-browning additives, 200 mg·L $^{-1}$ AC and 10 mg·L $^{-1}$ CA + AA (1:1) were best at controlling phenolic secretion from hypocotyl and cotyledon explants in callus induction medium. Hypocotyl explants were more suitable for callus initiation and organogenesis than cotyledon explants. Moreover, among twelve types of media tested for callus culture, the addition of 0.5 mg·L $^{-1}$ 2,4-dicholorophenoxy acetic acid (2,4-D) and 1.5 mg·L $^{-1}$ benzyladenine (BA) to the growth media was the most effective for hypocotyl explants, whereas MS + 1.5 mg·L $^{-1}$ α-naphthylacetic acid (NAA) and 0.5 mg·L $^{-1}$ 6-benzyloaminopurine (BAP) was effective for cotyledon explants. Improved shoot regeneration was achieved with 2 mg·L $^{-1}$ BAP and 0.1 mg·L $^{-1}$ indole butyric acid (IBA). The maximum number of strong and healthy roots was obtained on media that included 2 mg·L $^{-1}$ IBA and 200 mg·L $^{-1}$ AC. Rooted plantlets were successfully acclimatized in earthen pots, and the rooted plantlets had normal morphology and growth characteristics. The present study offers a potential solution for controlling phenolic secretion during okra regeneration using additives and evaluates the manipulation of plant growth regulators for effective regeneration of the Wufu okra cultivar.
Starting Page	503
Ending Page	513
Page Count	11
File Format	PDF
ISSN	22113452
Journal	Horticulture, Environment, and Biotechnology
Volume Number	58
Issue Number	5
e-ISSN	22113460
Language	English
Publisher	Korean Society for Horticultural Science
Publisher Date	2017-10-26
Publisher Place	Korea
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Life Sciences Plant Breeding/Biotechnology Plant Physiology Agriculture Plant Ecology
Content Type	Text
Resource Type	Article
Subject	Plant Science Horticulture Biotechnology

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
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3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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In vitro regeneration of Abelmoschus esculentus L. cv. Wufu: Influence of anti-browning additives on phenolic secretion and callus formation frequency in explants