NDLI: A simple method to determine amino acid leakage and germination capabilities from single radish (Raphanus sativus L.) and Chinese cabbage (Brassica rapa ssp. pekinensis) seeds

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Horticulture, Environment, and Biotechnology : Volume 54, Issue 2, April 2013

Horticulture, Environment, and Biotechnology : Volume 54, Issue 1, February 2013

Horticulture, Environment, and Biotechnology : Volume 53

Horticulture, Environment, and Biotechnology : Volume 52

A simple method to determine amino acid leakage and germination capabilities from single radish (Raphanus sativus L.) and Chinese cabbage (Brassica rapa ssp. pekinensis) seeds

Content Provider	Springer Nature Link
Author	Min, Tai Gi Hong, Ba Reun
Copyright Year	2013
Abstract	The viability of radish (Raphanus sativus L.) and Chinese cabbage (Brassica rapa ssp. pekinensis) seeds were evaluated using a seed leachate treated ninhydrin reagent that develops color from amino acid leakage. Three different cultivars of artificially aged radish and Chinese cabbage seeds were used for this purpose. The radish and Chinese cabbage seeds were individually soaked in distilled water and treated with the ninhydrin reagent. A clearly distinct purple color was observed for the treated seed leachate, with intensities that varied depending on the seed quality. Depending on the intensity, the color could be classified into four separate fractions: colorless, weak, medium or strong. Color absorbance values were classified into 9 fractions for radish seeds and 11 fractions for Chinese cabbage seeds. Subsequently, a germination test was performed with the seeds in any given fraction (classified by color intensity and absorbance) and correlations between germination and color intensity or absorbance were evaluated. In a test of three cultivars of artificially aged radish and Chinese cabbage seeds, the relationship between percent germinated and color intensity appeared to have a very high negative correlation in linear regression, with r$^{2}$ = 0.97 for radish seeds and r$^{2}$ = 0.95 for Chinese cabbage seeds. Similarly, the relationship between the percent germinated and the absorbance had a high negative correlation, with r$^{2}$ = 0.90 for radish seeds and r$^{2}$ = 0.94 for Chinese cabbage seeds. In conclusion, the simple detection of amino acid leakage with ninhydrin reagent was successful at assessing individual seed quality, as high correlations between germination and color intensity/absorbance were revealed.
Starting Page	249
Ending Page	256
Page Count	8
File Format	PDF
ISSN	22113452
Journal	Horticulture, Environment, and Biotechnology
Volume Number	54
Issue Number	3
e-ISSN	22113460
Language	English
Publisher	Springer Netherlands
Publisher Date	2013-07-05
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Life Sciences Plant Breeding/Biotechnology Plant Physiology Agriculture Plant Ecology
Content Type	Text
Resource Type	Article
Subject	Plant Science Horticulture Biotechnology

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Predicting germination probability of radish (Raphanus sativus L.), Chinese cabbage (Brassica rapa ssp. pekinensis), and cabbage (B. oleracea var. capitata L.) seeds via amino acid leakage parameters

Erratum to: Predicting germination probability of radish (Raphanus sativus L.), Chinese cabbage (Brassica rapa ssp. pekinensis), and cabbage (B. oleracea var. capitata L.) seeds via amino acid leakage parameters

Erratum to: Predicting germination probability of radish (Raphanus sativus L.), Chinese cabbage (Brassica rapa ssp. pekinensis), and cabbage (B. oleracea var. capitata L.) seeds via amino acid leakage parameters

Leakage of inorganic compounds from artificially-aged radish (Raphanus sativus L.) and Chinese cabbage (Brassica rapa ssp. pekinensis) seeds and use of phosphate leakage from single seed as a tool for assessing viability

A single amino acid residue substitution in BraA04g017190.3C, a histone methyltransferase, results in premature bolting in Chinese cabbage (Brassica rapa L. ssp. Pekinensis)

Developmental and germinative events can occur concurrently in precociously germinating Chinese cabbage (Brassica rapa ssp. Pekinensis) seeds

L-Cysteine Increases the Transformation Efficiency of Chinese Cabbage (Brassica rapa ssp. pekinensis)

Effects of Endogenous Salicylic Acid During Calcium Deficiency-Induced Tipburn in Chinese Cabbage (Brassica rapa L. ssp. pekinensis)

Loss of Function of the Carotenoid Isomerase Gene BrCRTISO Confers Orange Color to the Inner Leaves of Chinese Cabbage (Brassica rapa L. ssp. pekinensis)

A simple method to determine amino acid leakage and germination capabilities from single radish (Raphanus sativus L.) and Chinese cabbage (Brassica rapa ssp. pekinensis) seeds

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Predicting germination probability of radish (Raphanus sativus L.), Chinese cabbage (Brassica rapa ssp. pekinensis), and cabbage (B. oleracea var. capitata L.) seeds via amino acid leakage parameters

Erratum to: Predicting germination probability of radish (Raphanus sativus L.), Chinese cabbage (Brassica rapa ssp. pekinensis), and cabbage (B. oleracea var. capitata L.) seeds via amino acid leakage parameters

Erratum to: Predicting germination probability of radish (Raphanus sativus L.), Chinese cabbage (Brassica rapa ssp. pekinensis), and cabbage (B. oleracea var. capitata L.) seeds via amino acid leakage parameters

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A simple method to determine amino acid leakage and germination capabilities from single radish (Raphanus sativus L.) and Chinese cabbage (Brassica rapa ssp. pekinensis) seeds