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| Content Provider | Springer Nature Link |
|---|---|
| Author | Tomiyasu, Satoshi Miyamoto, Tomoyuki Mori, Michihiro Yaguchi, Takahiro Yakushiji, Hiromasa Ohno, Setsuyo Miyake, Yasuyuki Sakaguchi, Takuya Ueda, Masatsugu Ohno, Eiji |
| Copyright Year | 2013 |
| Abstract | Cancer stem cells (CSCs) possess the ability for self-renewal, differentiation, and tumorigenesis and play a role in cancer recurrence and metastasis. CSCs are usually sorted in analysis into side population (SP) cells using ultraviolet (UV) laser (350 nm) excitation; they cannot be stained with Hoechst 33342 because of their efflux ability. However, it is difficult to avoid cell damage using a UV laser. Therefore, we attempted to isolate CSCs using a violet laser (407 nm) excitation to avoid cellular DNA damage. We sorted SP cells and main population (MP) cells from a human endometrial cancer cell line using the FACSAria system equipped with a violet laser and analyzed the biological properties of these cells. SP cells exhibited drug efflux, self-renewal, differentiation abilities, and tumorigenicity. It was found that v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS) expression was significantly higher in SP cells than in MP cells. Our results suggest that CSCs exist in the SP fraction sorted using the FACSAria system equipped with a violet laser, which presents a useful tool to isolate small populations of viable putative CSCs from solid tumors and can be used to identify and characterize CSCs. |
| Starting Page | 36 |
| Ending Page | 42 |
| Page Count | 7 |
| File Format | |
| ISSN | 09147470 |
| Journal | Human Cell |
| Volume Number | 27 |
| Issue Number | 1 |
| e-ISSN | 17490774 |
| Language | English |
| Publisher | Springer Japan |
| Publisher Date | 2013-10-04 |
| Publisher Place | Tokyo |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Cancer stem cells Side population cells Violet laser Endometrial cancer KRAS Cell Biology Embryology Oncology Stem Cells Reproductive Medicine Cell Culture |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine Cancer Research |
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