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Formation of bipolar spindles with two centrosomes in tetraploid cells established from normal human fibroblasts

Content Provider	Springer Nature Link
Author	Ohshima, Susumu Seyama, Atsushi
Copyright Year	2012
Abstract	Tetraploid cells with unstable chromosomes frequently arise as an early step in tumorigenesis and lead to the formation of aneuploid cells. The mechanisms responsible for the chromosome instability of polyploid cells are not fully understood, although the supernumerary centrosomes in polyploid cells have been considered the major cause of chromosomal instability. The aim of this study was to examine the integrity of mitotic spindles and centrosomes in proliferative polyploid cells established from normal human fibroblasts. TIG-1 human fibroblasts were treated with demecolcine (DC) for 4 days to induce polyploidy, and the change in DNA content was monitored. Localization of centrosomes and mitotic spindles in polyploid mitotic cells was examined by immunohistochemistry and laser scanning cytometry. TIG-1 cells treated with DC became almost completely tetraploid at 2 weeks after treatment and grew at the same rate as untreated diploid cells. Most mitotic cells with 8C DNA content had only two centrosomes with bipolar spindles in established tetraploid cells, although they had four or more centrosomes with multipolar spindles at 3 days after DC treatment. The frequency of aneuploid cells increased as established tetraploid cells were propagated. These results indicate that tetraploid cells that form bipolar spindles with two centrosomes in mitosis can proliferate as diploid cells. These cells may serve as a useful model for studying the chromosome instability of polyploid cells.
Starting Page	78
Ending Page	85
Page Count	8
File Format	PDF
ISSN	09147470
Journal	Human Cell
Volume Number	25
Issue Number	3
e-ISSN	17490774
Language	English
Publisher	Springer Japan
Publisher Date	2012-06-14
Publisher Place	Japan
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Polyploidy Centrosome Mitotic spindle Chromosome instability Human fibroblast Reproductive Medicine Cell Biology Cell Culture Oncology Embryology Stem Cells
Content Type	Text
Resource Type	Article
Subject	Cell Biology Medicine Cancer Research

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