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| Content Provider | Springer Nature Link |
|---|---|
| Author | Talarczyk Desole, Joanna Kotwicka, Małgorzata Jendraszak, Magdalena Pawelczyk, Leszek Murawski, Marek Jędrzejczak, Piotr |
| Copyright Year | 2016 |
| Abstract | The aim of this study was to investigate the relationship between apoptotic markers present in human spermatozoa, namely phosphatidylserine translocation (PST) from the inner to the outer layer of the cytomembrane and the active form of caspase-3 (c3) versus the fertilizing potential of male gametes in conventional in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) models. A total of 116 male patients treated with their partners for infertility underwent basic semen analysis and an assessment of the presence of PST and the active c3 in sperm using flow cytometry. Forty patients underwent IVF, group A, while 76 patients underwent ICSI, group B. The fertilizing potential of the gametes was measured as the percentage of oocytes with pronuclei present after either procedure. PST and active c3 were identified in vital gametes, mainly in the midpiece area. Concentration, motility, morphology, and viability of spermatozoa strongly negatively correlated with both markers. In group A, a negative correlation between both markers and the success rate of conventional IVF was observed (r = −0.4, p = 0.04 for PST; r = −0.4, p = 0.02 for active c3, respectively). In group B, the success rate of ICSI did not correlate with either marker (r = −0.2, p = 0.85 for PST and r = 0.1, p = 0.51 for active c3). The two apoptotic markers localized in the sperm midpiece area may affect their function not only by decreasing basic andrologic parameters but also by reducing the probability of conception. Therefore, analysis of PST and active c3 in the sperm of patients undergoing infertility treatment should be recommended. |
| Starting Page | 67 |
| Ending Page | 75 |
| Page Count | 9 |
| File Format | |
| ISSN | 09147470 |
| Journal | Human Cell |
| Volume Number | 29 |
| Issue Number | 2 |
| e-ISSN | 17490774 |
| Language | English |
| Publisher | Springer Japan |
| Publisher Date | 2016-01-20 |
| Publisher Place | Tokyo |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Male infertility IVF ICSI Spermatozoa Phosphatidylserine translocation Caspase-3 Apoptotic markers Cell Biology Embryology Oncology Stem Cells Reproductive Medicine Cell Culture |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine Cancer Research |
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