NDLI: Up-regulation of lymphocyte antigen 6 complex expression in side-population cells derived from a human trophoblast cell line HTR-8/SVneo

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Up-regulation of lymphocyte antigen 6 complex expression in side-population cells derived from a human trophoblast cell line HTR-8/SVneo

Content Provider	Springer Nature Link
Author	Inagaki, Tetsunori Kusunoki, Soshi Tabu, Kouichi Okabe, Hitomi Yamada, Izumi Taga, Tetsuya Matsumoto, Akemi Makino, Shintaro Takeda, Satoru Kato, Kiyoko
Copyright Year	2015
Abstract	The continual proliferation and differentiation of trophoblasts are critical for the maintenance of pregnancy. It is well known that the tissue stem cells are associated with the development of tissues and pathologies. It has been demonstrated that side-population (SP) cells identified by fluorescence-activated cell sorting (FACS) are enriched with stem cells. The SP cells in HTR-8/SVneo cells derived from human primary trophoblast cells were isolated by FACS. HTR-8/SVneo-SP cell cultures generated both SP and non-SP (NSP) subpopulations. In contrast, NSP cell cultures produced NSP cells and failed to produce SP cells. These SP cells showed self-renewal capability by serial colony-forming assay. Microarray expression analysis using a set of HTR-8/SVneo-SP and -NSP cells revealed that SP cells overexpressed several stemness genes including caudal type homeobox2 (CDX2) and bone morphogenic proteins (BMPs), and lymphocyte antigen 6 complex locus D (LY6D) gene was the most highly up-regulated in HTR-8/SVneo-SP cells. LY6D gene reduced its expression in the course of a 7-day cultivation in differentiation medium. SP cells tended to reduce its fraction by treatment of LY6D siRNA indicating that LY6D had potential to maintain cell proliferation of HTR-8/SVneo-SP cells. On ontology analysis, epithelial–mesenchymal transition (EMT) pathway was involved in the up-regulated genes on microarray analysis. HTR-SVneo-SP cells showed enhanced migration. This is the first report that LY6D was important for the maintenance of HTR-8/SVneo-SP cells. EMT was associated with the phenotype of these SP cells.
Starting Page	10
Ending Page	21
Page Count	12
File Format	PDF
ISSN	09147470
Journal	Human Cell
Volume Number	29
Issue Number	1
e-ISSN	17490774
Language	English
Publisher	Springer Japan
Publisher Date	2015-07-30
Publisher Place	Tokyo
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Trophoblast Epithelial–mesenchymal transition HTR-8/SVneo LY6D Side population cell Cell Biology Embryology Oncology Stem Cells Reproductive Medicine Cell Culture
Content Type	Text
Resource Type	Article
Subject	Cell Biology Medicine Cancer Research

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Circadian variation of cell proliferation in HTR-8/SVneo cell line

Isolation and Characterization of Human Trophoblast Side-Population (SP) Cells in Primary Villous Cytotrophoblasts and HTR-8/SVneo Cell Line

The effects of progesterone on apoptosis in the human trophoblast-derived HTR-8/SV neo cells

Extracellular presence/release of galectins from HTR-8/SVneo extravillous trophoblast cells*

Extracellular presence/release of galectins from HTR-8/SVneo extravillous trophoblast cells*

Extracellular presence/release of galectins from HTR-8/SVneo extravillous trophoblast cells*

BMP9 maintains the phenotype of HTR-8/Svneo trophoblast cells by activating the SDF1/CXCR4 pathway

Isolation and Characterization of Human Trophoblast Side-Population (SP) Cells in Primary Villous Cytotrophoblasts and HTR-8/SVneo Cell Line

Serum depletion induces changes in protein expression in the trophoblast-derived cell line HTR-8/SVneo

Up-regulation of lymphocyte antigen 6 complex expression in side-population cells derived from a human trophoblast cell line HTR-8/SVneo

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