NDLI: Neuronal and glial differentiation following culture of the human embryonic cortical stem cells

Content Provider	Springer Nature Link
Author	Liang, Peng Zhao, Shiguang Kawmoto, Keiji Jin, Lianhong Liu, Enzhong
Copyright Year	2003
Abstract	To set up long-term in vitro culture system of the human neural stem cells (hNSC) and to study their biological properties.Human fetuses aged about 20 weeks following spontaneous abortion were adopted. A serum-free medium containing basic fibroblast growth factor and epidermal growth factor was used to make the hNSCs divide continuously in the culture. The growth curve of continually passaged cells was examined. The effects of long-term culture on the cell cycle, cell differentiation were analyzed. The cell cycles of these cells were analyzed using flow cytometry.The cells from the human embryonic cortical tissue could be maintained and propagated in the presence of growth factors. Neurospheres were generated continually. Only one month after the primary culture, the precursors could be effectively discarded. The cells could be cultured for ten months. The cells had an exponential, consistent growth. The cell cycle analysis indicated that the hNSCs maintained remarkable proliferation. Upon differentiation, the hNSCs gave rise to mature cells. The multi-lineage potential of differentiation after different passages remained unchanged.The hNSCs isolated from the human embryonic tissues retained their biological features after long-term culture in vitro.
Starting Page	151
Ending Page	156
Page Count	6
File Format	PDF
ISSN	09147470
Journal	Human Cell
Volume Number	16
Issue Number	3
e-ISSN	17490774
Language	English
Publisher	Springer-Verlag
Publisher Date	2012-08-06
Publisher Place	Tokyo
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	neural stem cell differentiation culture cell cycle embryo Cell Biology Embryology Oncology Stem Cells Reproductive Medicine Cell Culture
Content Type	Text
Resource Type	Article
Subject	Cell Biology Medicine Cancer Research

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