NDLI: An efficient in vitro regeneration system of fieldpea (Pisum sativum L.) via. shoot organogenesis

Content Provider	Springer Nature Link
Author	Das, Alok Kumar, Sumit Nandeesha, P. Yadav, Indu Singh Saini, Jyoti Chaturvedi, S. K. Datta, Subhojit
Copyright Year	2013
Abstract	In-vitro regeneration in fieldpea was achieved from immature embryonic axes and cotyledonary node explants of six genotypes on modified MS media supplemented with different concentration of plant growth regulators, 6-Benzylamino purine (BAP) and Naphthalene acetic acid (NAA). The best regeneration response, leading to multiple shoot formation efficiency (22.34 shoots/explant) was observed in the medium supplemented with 1.0 mg/L BAP and 0.2 mg/L NAA and best frequency (67.55 ± 4.74) was achieved on medium containing 2.0 mg/L BAP and 0.4 mg/L NAA. The shoots were subcultured on a medium supplemented with a combination of 1.0 mg/L GA$_{3}$, 2.0 mg/L BAP and 0.4 mg/L NAA, which resulted in elongation of 85 % of shoots. Rooting attempted from the elongated shoots, on half strength MS medium and supplemented with three different auxins IBA, IAA and NAA separately, exhibited similar results. Alternatively, micro-grafting of in vitro regenerated shoots onto pre-germinated root stocks raised in green house facility was attempted with high success rate (75 %). The grafted plants could be successfully hardened, fertigated with Hoagland solution and distilled water in a ratio of (1:10) for acclimatization and further development. All the genotypes tested, produced multiple shoots that could be established to mature fertile plant, hence, the medium combinations used were found to be genotype neutral.
Starting Page	184
Ending Page	189
Page Count	6
File Format	PDF
ISSN	09717811
Journal	Journal of Plant Biochemistry and Biotechnology
Volume Number	23
Issue Number	2
e-ISSN	09741275
Language	English
Publisher	Springer India
Publisher Date	2013-03-22
Publisher Place	India
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Pisum sativum L. Organogenesis Regeneration Auxins Cytokinins Micro-grafting Life Sciences Plant Biochemistry Protein Science Receptors Cell Biology
Content Type	Text
Resource Type	Article
Subject	Plant Science Biotechnology Agronomy and Crop Science

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