NDLI: Enhanced Alkaloid Production in Callus Cultures of Heliotropium indicum L and Regeneration of Plantlets

Content Provider	Springer Nature Link
Author	Datta, Aniruddha Narula, Alka Bansal, K. C. Srivastava, P. S.
Copyright Year	2003
Abstract	Heliotropium indicum L (Boraginaceae) contains the anticancer pyrrolizidine alkaloid, indicine-N-oxide (INO). To study the yield of INO as a function of plant development, plantlets were regenerated in vitro from nodal and hypocotyl explants and also from hypocotyl callus, on Murashige and Skoog’s (MS) medium supplemented with 1-naphthaleneacetic acid (NAA), 6-benzylaminopurine (BAP), asparagine (Asp) and glutamine (Glu). The regenerated plantlets were rooted on MS supplemented with Glu or gibberellic acid (GA$_{3}$). While 5-week-old seedlings showed a high amount of INO (0.12% dry wt) that depleted gradually as the plants attained maturity and flowered, fast growing callus produced a much higher yield of INO (0.32% dry wt). As the callus cultures differentiated into shoots and subsequently into plantlets, the INO content decreased to about 0.2% dry wt. It appears that INO is the primary product of pyrrolizidine alkaloid biosynthetic pathway in rapidly growing meristematic tissue of Heliotropium indicum, later reduced to its alkaloidal base and reallocated to other tissues. The article includes an efficient micropropagation method for Heliotropium indicum.
Starting Page	139
Ending Page	142
Page Count	4
File Format	PDF
ISSN	09717811
Journal	Journal of Plant Biochemistry and Biotechnology
Volume Number	12
Issue Number	2
e-ISSN	09741275
Language	English
Publisher	Springer India
Publisher Date	2012-12-30
Publisher Place	New Delhi
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Heliotropium indicum indicine-N-oxide pyrrolizidine alkaloids regeneration Life Sciences Plant Biochemistry Protein Science Receptors Cell Biology
Content Type	Text
Resource Type	Article
Subject	Plant Science Biotechnology Agronomy and Crop Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Tropane Alkaloids from Callus Cultures, Differentiated and Shoots of Hyoscyamus muticus L.

Regeneration of Plantlets via Callus Cultures in Celastrus paniculatus Willd-A Rare Endangered Medicinal Plant

Pyrrolizidine alkaloids from Heliotropium indicum

In vitro organogenesis from internode derived callus cultures of Capsicum annuum L.

Induced Caulogenesis in Long-term Callus Cultures of Rosrnarinus officinalis L

Selective uptake of pyrrolizidine N-oxides by cell suspension cultures from pyrrolizidine alkaloid producing plants.

Regeneration of Heracleum candicans Wall Plants from Callus Cultures through Organogenesis

Callus Formation and Plant Regeneration from Cultured Embryos of Diploid and Tetraploid Winter Ryes (Secale cereale L)

Enhanced production of withaferin-A in shoot cultures of Withania somnifera (L) Dunal

Enhanced Alkaloid Production in Callus Cultures of Heliotropium indicum L and Regeneration of Plantlets

Similar Documents

Tropane Alkaloids from Callus Cultures, Differentiated and Shoots of Hyoscyamus muticus L.

Regeneration of Plantlets via Callus Cultures in Celastrus paniculatus Willd-A Rare Endangered Medicinal Plant

Pyrrolizidine alkaloids from Heliotropium indicum

In vitro organogenesis from internode derived callus cultures of Capsicum annuum L.

Induced Caulogenesis in Long-term Callus Cultures of Rosrnarinus officinalis L

Selective uptake of pyrrolizidine N-oxides by cell suspension cultures from pyrrolizidine alkaloid producing plants.

Regeneration of Heracleum candicans Wall Plants from Callus Cultures through Organogenesis

Callus Formation and Plant Regeneration from Cultured Embryos of Diploid and Tetraploid Winter Ryes (Secale cereale L)

Enhanced production of withaferin-A in shoot cultures of Withania somnifera (L) Dunal

Enhanced Alkaloid Production in Callus Cultures of Heliotropium indicum L and Regeneration of Plantlets