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| Content Provider | Springer Nature Link |
|---|---|
| Author | Rajwanshi, Ravi Sarin, Neera Bhalla |
| Copyright Year | 2012 |
| Abstract | The use of antibiotic and herbicide resistance based negative selection in plant transformation experiments remains a major impediment in the acceptance of transgenic crops. To overcome this, Positech selection system involving the use of phosphomannose isomerase (pmi) gene from Escherichia coli and mannose as selection agent was exploited for the selection of transgenic Brassica juncea L. cv. Varuna. The transgenic plants were generated by transformation with Agrobacterium tumefaciens harbouring the pmi gene driven by a constitutive Cestrum leaf curling viral promoter. Supplementing the selection medium with 0.09 gl$^{-1}$ mannose and 5 gl$^{-1}$ glucose provided the optimal condition for the selection of transformed explants. Stable integration and expression of pmi gene was confirmed by Southern and northern blot analysis, respectively. Our results show that the pmi gene driven by the constitutive Cestrum leaf curling viral promoter can be successfully used for positive selection in transformation of B.juncea, an important agronomic oil-seed crop, and that a combination of mannose and glucose rather than mannose alone is more suitable for the selection. To the best of our knowledge, Positech system has not been used so far in transformation of Brassica juncea. |
| Starting Page | 214 |
| Ending Page | 221 |
| Page Count | 8 |
| File Format | |
| ISSN | 09717811 |
| Journal | Journal of Plant Biochemistry and Biotechnology |
| Volume Number | 22 |
| Issue Number | 2 |
| e-ISSN | 09741275 |
| Language | English |
| Publisher | Springer-Verlag |
| Publisher Date | 2012-09-08 |
| Publisher Place | India |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Brassica juncea Phosphomannose isomerase (pmi) POSITECH system Transgenics Cestrum leaf curling viral promoter Life Sciences Plant Biochemistry Protein Science Receptors Cell Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Plant Science Biotechnology Agronomy and Crop Science |
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