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| Content Provider | Springer Nature Link |
|---|---|
| Author | Yadav, Teena Kothari, S. L. Kachhwaha, Sumita |
| Copyright Year | 2011 |
| Abstract | A protocol for plant regeneration in Indian cultivars of barley (Hordeum vulgare L.) has been developed using mature embryo culture. The influence of various auxins 2,4-D (2,4-dichlorophenoxyacetic acid), Dicamba (3,6-dichloro-o-anisic acid) and Picloram (4-amino-3,5,6-trichloropicolinic acid) on the callus induction and subsequent plant regeneration revealed highest percent of callus induction form cultivar (cv) BL 2 on MSB$_{5}$ medium (MS salts + B$_{5}$ vitamins) supplemented with 6 mg l$^{−1}$ Picloram, but maximum number of shoot buds (6–13) were regenerated on MSB$_{5}$ medium containing 0.5 mg l$^{−1}$ Picloram. Regenerated shoots were rooted on half-strength MSB$_{5}$ medium. Plantlets were successfully transferred to soil and grown to maturity in greenhouse. The effect of copper sulphate revealed significant improvement in callus induction and plant regeneration when the concentration of CuSO$_{4}$ was increased to 3 μM (30 times higher than normal MS medium) for cv BL 2. Regeneration potential differed for different cultivars of barley used, with highest for cv BL 2 and lowest for cv BH 924. We conclude that the Indian barley genotypes exhibit plant regeneration from mature embryo cultures. The protocol has potential application in barley improvement through genetic engineering. |
| Starting Page | 166 |
| Ending Page | 172 |
| Page Count | 7 |
| File Format | |
| ISSN | 09717811 |
| Journal | Journal of Plant Biochemistry and Biotechnology |
| Volume Number | 20 |
| Issue Number | 2 |
| e-ISSN | 09741275 |
| Language | English |
| Publisher | Springer-Verlag |
| Publisher Date | 2011-04-09 |
| Publisher Place | India |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Barley (Hordeum vulgare) Mature embryo Callus induction Plant regeneration MSB$_{5}$ medium Copper sulphate Protein Science Receptors Cell Biology Life Sciences Plant Biochemistry |
| Content Type | Text |
| Resource Type | Article |
| Subject | Plant Science Biotechnology Agronomy and Crop Science |
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