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| Content Provider | Springer Nature Link |
|---|---|
| Author | Diniz, Marina Gonçalves Gomes, Carolina Cavaliéri Castro, Wagner Henriques Guimarães, André Luiz Sena Paula, Alfredo Maurício Batista Amm, Hope Ren, Chanchung MacDougall, Mary Gomez, Ricardo Santiago |
| Copyright Year | 2012 |
| Abstract | A keratocystic odontogenic tumor (KCOT) is a benign destructive recurrent odontogenic cystic neoplasm. The microRNAs (miRNAs) miR-15a and miR-16-1 function as negative regulators of the anti-apoptotic gene BCL2 at the post-transcriptional level. Notably, high Bcl-2 immunoexpression is found in the epithelial lining of KCOTs, while the loss of Bcl-2 immunopositive cells is observed in marsupialized cysts. The purpose of this study was to investigate whether the transcription of miR-15a and miR-16-1 is altered in KCOTs and whether it is associated with BCL2 gene expression in such lesions.Using qRT-PCR and immunohistochemical analyses, we examined miR-15a/16-1 and BCL2 gene expression in KCOTs. The impact of miR-15a/16-1 expression on BCL2 gene translation was investigated by in vitro studies using primary KCOT culture cells.Using qRT-PCR, we observed miR-15a and/or miR-16-1 downregulation in the majority of the KCOT samples (24 of 28). We also observed higher BCL2 mRNA expression in 19 of 20 KCOT frozen samples and moderate to high Bcl-2 immunopositivity in the basal layer cells of 16 of 18 paraffin embedded KCOTs (median: 42.6 %). In vitro over-expression of miR-15a/16-1 in human KCOT-1 primary cell cultures resulted in a decrease in Bcl-2 protein expression. Furthermore, all five paired KCOTs collected before and after marsupialization treatment exhibited an increase in miR-15a after the procedure.Our results suggest that KCOT neoplastic cells exhibit an anti-apoptotic profile that may be related to lower miR-15a/16-1 expression. Additionally, we demonstrated that miRNA expression increases after marsupialization, implicating an etiological and therapeutic role of miRNAs in KCOT. |
| Starting Page | 285 |
| Ending Page | 291 |
| Page Count | 7 |
| File Format | |
| ISSN | 22113428 |
| Journal | Cellular Oncology |
| Volume Number | 35 |
| Issue Number | 4 |
| e-ISSN | 22113436 |
| Language | English |
| Publisher | Springer Netherlands |
| Publisher Date | 2012-06-09 |
| Publisher Institution | International Society for Cellular Oncology |
| Publisher Place | Dordrecht |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Odontogenic keratocyst keratocystic odontogenic tumor BCL2 microRNA miR15a/16-1 Pathology Biomedicine general Oncology Cancer Research |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cancer Research Molecular Medicine Oncology |
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