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| Content Provider | Springer Nature Link |
|---|---|
| Author | Sen, Sudip Kumar Raut, Smita Satpathy, Soumya Bandyopadhyay, Bidyut Mohapatra, Pradeep Kumar Das Raut, Sangeeta |
| Copyright Year | 2014 |
| Abstract | The nature of microbial communities and their relation to enzyme activities in the Taptapani hot spring Odisha, India, is a neglected area of investigation. To address this, the microbial diversity, enzyme activities and the physicochemical factors of the hot spring water were studied. Organisms were identified using the 16S rDNA, 28S rDNA and 18S region of rDNA for bacteria, fungi and algae, respectively, after amplification by the polymerase chain reaction. Phylogenetic analysis of the sequenced gel electrophoresis bands revealed a great diversity of microorganisms. Based on morphology, nine groups of bacteria, three groups of fungi and four groups of algae were dominant during all seasons. The growth behavior of the selected isolates was studied at different elevated temperatures which revealed that all ten isolates (six bacteria, three fungi and one alga) were able to grow in the temperature range of 50–80°C and at pH 4–9. Maximum temperature and pH for the amylase activity were found to be 80 and 9°C, respectively, while an optimum amylase activity was observed at 60°C and pH 9. Considerable activity was observed at both acidic and alkaline pH (4.0–10.0), suggesting wide technical applications for these enzymes and indicating that these enzymes are novel. |
| Starting Page | 51 |
| Ending Page | 61 |
| Page Count | 11 |
| File Format | |
| ISSN | 13198025 |
| Journal | Arabian Journal for Science and Engineering |
| Volume Number | 40 |
| Issue Number | 1 |
| e-ISSN | 21914281 |
| Language | English |
| Publisher | Springer Berlin Heidelberg |
| Publisher Date | 2014-12-07 |
| Publisher Place | Berlin, Heidelberg |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Biodiversity Thermophilic Amylase Taptapani rDNA Engineering Science |
| Content Type | Text |
| Resource Type | Article |
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