NDLI: Flame Atmospheric Pressure Chemical Ionization Coupled with Negative Electrospray Ionization Mass Spectrometry for Ion Molecule Reactions

Content Provider	Springer Nature Link
Author	Cheng, Sy Chyi Bhat, Suhail Muzaffar Shiea, Jentaie
Copyright Year	2017
Abstract	Flame atmospheric pressure chemical ionization (FAPCI) combined with negative electrospray ionization (ESI) mass spectrometry was developed to detect the ion/molecule reactions (IMRs) products between nitric acid (HNO$_{3}$) and negatively charged amino acid, angiotensin I (AI) and angiotensin II (AII), and insulin ions. Nitrate and HNO$_{3}$-nitrate ions were detected in the oxyacetylene flame, suggesting that a large quantity of nitric acid (HNO$_{3}$) was produced in the flame. The HNO$_{3}$ and negatively charged analyte ions produced by a negative ESI source were delivered into each arm of a Y-shaped stainless steel tube where they merged and reacted. The products were subsequently characterized with an ion trap mass analyzer attached to the exit of the Y-tube. HNO$_{3}$ showed the strongest affinity to histidine and formed (M$_{histidine}$-H+HNO$_{3}$)$^{–}$ complex ions, whereas some amino acids did not react with HNO$_{3}$ at all. Reactions between HNO$_{3}$ and histidine residues in AI and AII resulted in the formation of dominant [M$_{AI}$-H+(HNO$_{3}$)]$^{-}$ and [M$_{AII}$-H+(HNO$_{3}$)]$^{–}$ ions. Results from analyses of AAs and insulin indicated that HNO$_{3}$ could not only react with basic amino acid residues, but also with disulfide bonds to form [M-3H+(HNO$_{3}$)$_{n}$]$^{3-}$ complex ions. This approach is useful for obtaining information about the number of basic amino acid residues and disulfide bonds in peptides and proteins. Graphical Abstract ᅟ
Starting Page	1473
Ending Page	1481
Page Count	9
File Format	PDF
ISSN	10440305
Journal	Journal of The American Society for Mass Spectrometry
Volume Number	28
Issue Number	7
e-ISSN	18791123
Language	English
Publisher	Springer US
Publisher Date	2017-05-15
Publisher Institution	The American Society for Mass Spectrometry
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	HNO$_{3}$ Ion/molecule reactions Histidine Flame atmospheric pressure chemical ionization Negative electrospray ionization Y-tube Analytical Chemistry Biotechnology Organic Chemistry Proteomics Bioinformatics
Content Type	Text
Resource Type	Article
Subject	Spectroscopy Structural Biology

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