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| Content Provider | Springer Nature Link |
|---|---|
| Author | Keppel, Theodore R. Jacques, Martin E. Young, Robert W. Ratzlaff, Kenneth L. Weis, David D. |
| Copyright Year | 2011 |
| Abstract | Loss of deuterium label during the LC step in amide hydrogen/deuterium exchange mass spectrometry (H/D-MS) is minimized by maintaining an acidic mobile phase pH and low temperature (pH 2.5, 0 °C). Here we detail the construction and performance of a low-cost, thermoelectrically refrigerated enclosure to house high-performance liquid chromatography (HPLC) components and cool mobile phases. Small volume heat exchangers rapidly decrease mobile phase temperature and keep the temperature stable to ±0.2 °C. Using a superficially porous reversed-phase column, we obtained excellent chromatographic performance in the separation of peptides with a median peak width of 4.4 s. Average deuterium recovery was 80.2% with an average relative precision of 0.91%. |
| Starting Page | 1472 |
| Ending Page | 1476 |
| Page Count | 5 |
| File Format | |
| ISSN | 10440305 |
| Journal | Journal of The American Society for Mass Spectrometry |
| Volume Number | 22 |
| Issue Number | 8 |
| e-ISSN | 18791123 |
| Language | English |
| Publisher | Springer-Verlag |
| Publisher Date | 2011-05-15 |
| Publisher Institution | The American Society for Mass Spectrometry |
| Publisher Place | New York |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | H/D exchange Back-exchange Refrigeration Amide hydrogen/deuterium exchange Organic Chemistry Proteomics Bioinformatics Biotechnology Analytical Chemistry |
| Content Type | Text |
| Resource Type | Article |
| Subject | Spectroscopy Structural Biology |
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