NDLI: Transformation of [M+2H]$^{2+}$ Peptide Cations to [M – H]$^{+}$, [M+H+O]$^{+}$, and M$^{+•}$ Cations via Ion/Ion Reactions: Reagent Anions Derived from Persulfate

Content Provider	Springer Nature Link
Author	Pilo, Alice L. Bu, Jiexun McLuckey, Scott A.
Copyright Year	2015
Abstract	The gas-phase oxidation of doubly protonated peptides is demonstrated here using ion/ion reactions with a suite of reagents derived from persulfate. Intact persulfate anion (HS$_{2}$O$_{8}$ $^{–}$), peroxymonosulfate anion (HSO$_{5}$ $^{–}$), and sulfate radical anion (SO$_{4}$ $^{–•}$) are all either observed directly upon negative nanoelectrospray ionization (nESI) or easily obtained via beam-type collisional activation of persulfate into the mass spectrometer. Ion/ion reactions between each of these reagents and doubly protonated peptides result in the formation of a long-lived complex. Collisional activation of the complex containing a peroxymonosulfate anion results in oxygen transfer from the reagent to the peptide to generate the [M+H+O]$^{+}$ species. Activation of the complex containing intact persulfate anion either results in oxygen transfer to generate the [M+H+O]$^{+}$ species or abstraction of two hydrogen atoms and a proton to generate the [M – H]$^{+}$ species. Activation of the complex containing sulfate radical anion results in abstraction of one hydrogen atom and a proton to form the peptide radical cation, [M]$^{+•}$. This suite of reagents allows for the facile transformation of the multiply protonated peptides obtained via nESI into a variety of oxidized species capable of providing complementary information about the sequence and structure of the peptide. Graphical Abstract ᅟ
Starting Page	1103
Ending Page	1114
Page Count	12
File Format	PDF
ISSN	10440305
Journal	Journal of The American Society for Mass Spectrometry
Volume Number	26
Issue Number	7
e-ISSN	18791123
Language	English
Publisher	Springer US
Publisher Date	2015-05-06
Publisher Institution	The American Society for Mass Spectrometry
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Ion/ion reactions Multiply protonated peptide Persulfate Sulfate radical anion Peroxymonosulfate Analytical Chemistry Biotechnology Organic Chemistry Proteomics Bioinformatics
Content Type	Text
Resource Type	Article
Subject	Spectroscopy Structural Biology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Transformation of [M+2H]2+ Peptide Cations to [M – H]+, [M+H+O]+, and M+• Cations via Ion/Ion Reactions: Reagent Anions Derived from Persulfate

Transformation of [M+2H]2+ Peptide Cations to [M-H]+, [M+H+O]+, and M+• Cations via Ion/Ion Reactions: Reagent Anions Derived from Persulfate

Benzoylnitrene radical anion: A new reagent for the generation of M-2H anions

The Generation of Dehydroalanine Residues in Protonated Polypeptides: Ion/Ion Reactions for Introducing Selective Cleavages

Reagent Cluster Anions for Multiple Gas-Phase Covalent Modifications of Peptide and Protein Cations

Gas-phase ion/ion reactions of transition metal complex cations with multiply charged oligodeoxynucleotide anions

Ion/Ion Reactions with “Onium” Reagents: An Approach for the Gas-phase Transfer of Organic Cations to Multiply-Charged Anions

Benzoylnitrene radical anion: A new reagent for the generation of M-2H anions

Selective Removal of Alkali Metal Cations from Multiply-Charged Ions via Gas-Phase Ion/Ion Reactions Using Weakly Coordinating Anions

Transformation of [M+2H]$^{2+}$ Peptide Cations to [M – H]$^{+}$, [M+H+O]$^{+}$, and M$^{+•}$ Cations via Ion/Ion Reactions: Reagent Anions Derived from Persulfate

Similar Documents

Transformation of [M+2H]2+ Peptide Cations to [M – H]+, [M+H+O]+, and M+• Cations via Ion/Ion Reactions: Reagent Anions Derived from Persulfate

Transformation of [M+2H]2+ Peptide Cations to [M-H]+, [M+H+O]+, and M+• Cations via Ion/Ion Reactions: Reagent Anions Derived from Persulfate

Benzoylnitrene radical anion: A new reagent for the generation of M-2H anions

The Generation of Dehydroalanine Residues in Protonated Polypeptides: Ion/Ion Reactions for Introducing Selective Cleavages

Reagent Cluster Anions for Multiple Gas-Phase Covalent Modifications of Peptide and Protein Cations

Gas-phase ion/ion reactions of transition metal complex cations with multiply charged oligodeoxynucleotide anions

Ion/Ion Reactions with “Onium” Reagents: An Approach for the Gas-phase Transfer of Organic Cations to Multiply-Charged Anions

Benzoylnitrene radical anion: A new reagent for the generation of M-2H anions

Selective Removal of Alkali Metal Cations from Multiply-Charged Ions via Gas-Phase Ion/Ion Reactions Using Weakly Coordinating Anions

Transformation of [M+2H]$^{2+}$ Peptide Cations to [M – H]$^{+}$, [M+H+O]$^{+}$, and M$^{+•}$ Cations via Ion/Ion Reactions: Reagent Anions Derived from Persulfate