NDLI: Overtone Mobility Spectrometry: Part 4. OMS-OMS Analyses of Complex Mixtures

Content Provider	Springer Nature Link
Author	Kurulugama, Ruwan T. Nachtigall, Fabiane M. Valentine, Stephen J. Clemmer, David E.
Copyright Year	2011
Abstract	A new, two-dimensional overtone mobility spectrometry (OMS-OMS) instrument is described for the analysis of complex peptide mixtures. OMS separations are based on the differences in mobilities of ions in the gas phase. The method utilizes multiple drift regions with modulated drift fields such that only ions with appropriate mobilities are transmitted to the detector. Here we describe a hybrid OMS-OMS combination that utilizes two independently operated OMS regions that are separated by an ion activation region. Mobility-selected ions from the first OMS region are exposed to energizing collisions and may undergo structural transitions before entering the second OMS region. This method generates additional peak capacity and allows for higher selectivity compared with the one-dimensional OMS method. We demonstrate the approach using a three-protein tryptic digest spiked with the peptide Substance P. The [M + 3H]$^{3+}$ ion from Substance P can be completely isolated from other components in this complex mixture prior to introduction into the mass spectrometer.
Starting Page	2049
Ending Page	2060
Page Count	12
File Format	PDF
ISSN	10440305
Journal	Journal of The American Society for Mass Spectrometry
Volume Number	22
Issue Number	11
e-ISSN	18791123
Language	English
Publisher	Springer-Verlag
Publisher Date	2011-08-09
Publisher Institution	The American Society for Mass Spectrometry
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Ion mobility spectrometry Overtone mobility spectrometry Mass spectrometry Complex mixture analysis Bioinformatics Organic Chemistry Proteomics Biotechnology Analytical Chemistry
Content Type	Text
Resource Type	Article
Subject	Spectroscopy Structural Biology

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