NDLI: Characterization of acylphosphatidylglycerols from salmonella typhimurium by tandem mass spectrometry with electrospray ionization

Content Provider	Springer Nature Link
Author	Hsu, Fong Fu Turk, John Shi, Yixin Groisman, Eduardo A.
Copyright Year	2004
Abstract	Acylphosphatidylglycerol (Acyl-PG), a polar lipid class containing three fatty acyl groups, was isolated from Salmonella bacteria and characterized by tandem quadrupole and quadrupole ion-trap mass spectrometric methods with electrospray ionization. The structural characterization of the acyl-PG with various acyl groups (A-B/C-PG, where A≠B≠C) is based on the findings that the carboxylate anions (R$_{x}$CO 2 − ) arising from sn-2 (R$_{2}$CO$_{2/−}$) is more abundant than that arising from sn-3′ (R$_{3′}$CO 2 − ), which is much more abundant than that arising from sn-1 (R$_{1}$CO$_{2/−}$). This information provides a simple method for determination of the fatty acyl moieties and their positions in the molecule. The structural identification of the molecule can also be achieved by the findings that the fragment ion reflecting the ketene loss at sn-2 is more prominent than that reflecting the acid loss (i. e. , [M−H - R′$_{2}$CH=CO]$^{−}$>[M−H - R$_{2}$CO$_{2}$H]$^{−}$), while the ion arising from acid loss at sn-1 or sn-3′ is, respectively, more abundant than the corresponding ketene loss (i. e. , [M−H -R$_{1}$CO$_{2}$H]$^{−}$>[M−H - R′$_{1}$CH=CO]$^{−}$; [M−H - R$_{3′}$CO$_{2}$H]$^{−}$>[M−H -R′$_{3′}$CH=CO]$^{−}$). The identity of the acyl moiety at sn-3′ can be confirmed by an acyl-glycerophosphate anion observed in the product-ion spectrum obtained with a triple-stage quadrupole (TSQ) instrument, but not in that obtained with an ion-trap mass spectrometer (ITMS). However, the MS$^{2}$-spectrum obtained with an ITMS is featured by the ion series that abundances of [M−H -R′$_{2}$CH=CO - R$_{3}$CO$_{2}$H - 74]$^{−}$>[M−H - R′$_{2}$CH=CO - R$_{1}$CO$_{2}$H - 74]$^{−}$≫[M−H -R′$_{1(or 3′)}$CH=CO - R$_{3′(or 1)}$CO$_{2}$H - 74]$^{−}$. This information also facilitates structural elucidation of the acyl-PG subclass that contains various acyl substituents. Structural identifications of molecular species having two identical fatty acyl substituents at sn-1, sn-2, or sn-3′ or consisting of more than one isomeric structures are also demonstrated. The identities of the minor isomeric species in the molecules can be revealed by the aforementioned structural information arising from the various ion series combined.
Starting Page	1
Ending Page	11
Page Count	11
File Format	PDF
ISSN	10440305
Journal	Journal of The American Society for Mass Spectrometry
Volume Number	15
Issue Number	1
e-ISSN	18791123
Language	English
Publisher	Springer-Verlag
Publisher Date	2004-01-01
Publisher Institution	The American Society for Mass Spectrometry
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Analytical Chemistry Biotechnology Organic Chemistry Proteomics Bioinformatics
Content Type	Text
Resource Type	Article
Subject	Spectroscopy Structural Biology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Characterization of acylphosphatidylglycerols from salmonella typhimurium by tandem mass spectrometry with electrospray ionization

Characterization of inositol phosphorylceramides from Leishmania major by tandem mass spectrometry with electrospray ionization

Structural determination of sphingomyelin by tandem mass spectrometry with electrospray ionization

Structural characterization of cardiolipin by tandem quadrupole and multiple-stage quadrupole ion-trap mass spectrometry with electrospray ionization

Characterization of ceramides by low energy collisional-activated dissociation tandem mass spectrometry with negative-ion electrospray ionization

Electrospray Ionization Mass Spectrometry of Palladium(II) Quinolinylaminophosphonate Complexes

Studies on phosphatidylglycerol with triple quadrupole tandem mass spectrometry with electrospray ionization: Fragmentation processes and structural characterization

Structural analysis of saponins from medicinal herbs using electrospray ionization tandem mass spectrometry

Electrospray ionization tandem mass spectrometry of glycerophosphoethanolamine plasmalogen phospholipids

Characterization of acylphosphatidylglycerols from salmonella typhimurium by tandem mass spectrometry with electrospray ionization

Similar Documents

Characterization of acylphosphatidylglycerols from salmonella typhimurium by tandem mass spectrometry with electrospray ionization

Characterization of inositol phosphorylceramides from Leishmania major by tandem mass spectrometry with electrospray ionization

Structural determination of sphingomyelin by tandem mass spectrometry with electrospray ionization

Structural characterization of cardiolipin by tandem quadrupole and multiple-stage quadrupole ion-trap mass spectrometry with electrospray ionization

Characterization of ceramides by low energy collisional-activated dissociation tandem mass spectrometry with negative-ion electrospray ionization

Electrospray Ionization Mass Spectrometry of Palladium(II) Quinolinylaminophosphonate Complexes

Studies on phosphatidylglycerol with triple quadrupole tandem mass spectrometry with electrospray ionization: Fragmentation processes and structural characterization

Structural analysis of saponins from medicinal herbs using electrospray ionization tandem mass spectrometry

Electrospray ionization tandem mass spectrometry of glycerophosphoethanolamine plasmalogen phospholipids

Characterization of acylphosphatidylglycerols from salmonella typhimurium by tandem mass spectrometry with electrospray ionization