NDLI: Formation and Dissociation of the Interstrand i-Motif by the Sequences d(X$_{n}$C$_{4}$Y$_{m}$) Monitored with Electrospray Ionization Mass Spectrometry

Content Provider	Springer Nature Link
Author	Cao, Yanwei Qin, Yujiao Bruist, Michael Gao, Shang Wang, Bing Wang, Huixin Guo, Xinhua
Copyright Year	2015
Abstract	Formation and dissociation of the interstrand i-motifs by DNA with the sequence d(X$_{n}$C$_{4}$Y$_{m}$) (X and Y represent thymine, adenine, or guanine, and n, m range from 0 to 2) are studied with electrospray ionization mass spectrometry (ESI-MS), circular dichroism (CD), and UV spectrophotometry. The ion complexes detected in the gas phase and the melting temperatures (T$_{m}$) obtained in solution show that a non-C base residue located at 5′ end favors formation of the four-stranded structures, with T > A > G for imparting stability. Comparatively, no rule is found when a non-C base is located at the 3′ end. Detection of penta- and hexa-stranded ions indicates the formation of i-motifs with more than four strands. In addition, the i-motifs seen in our mass spectra are accompanied by single-, double-, and triple-stranded ions, and the trimeric ions were always less abundant during annealing and heat-induced dissociation process of the DNA strands in solution (pH = 4.5). This provides a direct evidence of a strand-by-strand formation and dissociation pathway of the interstrand i-motif and formation of the triple strands is the rate-limiting step. In contrast, the trimeric ions are abundant when the tetramolecular ions are subjected to collision-induced dissociation (CID) in the gas phase, suggesting different dissociation behaviors of the interstrand i-motif in the gas phase and in solution. Furthermore, hysteretic UV absorption melting and cooling curves reveal an irreversible dissociation and association kinetic process of the interstrand i-motif in solution. Graphical Abstract ᅟ
Starting Page	994
Ending Page	1003
Page Count	10
File Format	PDF
ISSN	10440305
Journal	Journal of The American Society for Mass Spectrometry
Volume Number	26
Issue Number	6
e-ISSN	18791123
Language	English
Publisher	Springer US
Publisher Date	2015-04-11
Publisher Institution	The American Society for Mass Spectrometry
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Interstrand i-motif Terminal non-C base effect I-motif formation and dissociation pathways Mass spectrometry UV melting curves Analytical Chemistry Biotechnology Organic Chemistry Proteomics Bioinformatics
Content Type	Text
Resource Type	Article
Subject	Spectroscopy Structural Biology

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