NDLI: High-Resolution Ambient MS Imaging of Negative Ions in Positive Ion Mode: Using Dicationic Reagents with the Single-Probe

Content Provider	Springer Nature Link
Author	Rao, Wei Pan, Ning Tian, Xiang Yang, Zhibo
Copyright Year	2015
Abstract	We have used the Single-probe, a miniaturized sampling device utilizing in-situ surface microextraction for ambient mass spectrometry (MS) analysis, for the high resolution MS imaging (MSI) of negatively charged species in the positive ionization mode. Two dicationic compounds, 1,5-pentanediyl-bis(1-butylpyrrolidinium) difluoride [C$_{5}$(bpyr)$_{2}$F$_{2}$] and 1,3-propanediyl-bis(tripropylphosphonium) difluoride [C$_{3}$(triprp)$_{2}$F$_{2}$], were added into the sampling solvent to form 1+ charged adducts with the negatively charged species extracted from tissues. We were able to detect 526 and 322 negatively charged species this way using [C$_{5}$(bpyr)$_{2}$F$_{2}$] and [C$_{3}$(triprp)$_{2}$F$_{2}$], respectively, including oleic acid, arachidonic acid, and several species of phosphatidic acid, phosphoethanolamine, phosphatidylserine, phosphatidylglycerol, phosphatidylinositol, and others. In conjunction with the identification of the non-adduct cations, we have tentatively identified a total number of 1200 and 828 metabolites from mouse brain sections using [C$_{5}$(bpyr)$_{2}$F$_{2}$] and [C$_{3}$(triprp)$_{2}$F$_{2}$], respectively, through high mass accuracy measurements (mass error <5 ppm); MS/MS analyses were also performed to verify the identity of selected species. In addition to the high mass accuracy measurement, we were able to generate high spatial resolution (~17 μm) MS images of mouse brain sections. Our study demonstrated that utilization of dicationic compounds in the surface microextraction with the Single-probe device can perform high mass and spatial resolution ambient MSI measurements of broader types of compounds in tissues. Other reagents can be potentially used with the Single-probe device for a variety of reactive MSI studies to enable the analysis of species that are previously intractable. Graphical Abstract ᅟ
Starting Page	124
Ending Page	134
Page Count	11
File Format	PDF
ISSN	10440305
Journal	Journal of The American Society for Mass Spectrometry
Volume Number	27
Issue Number	1
e-ISSN	18791123
Language	English
Publisher	Springer US
Publisher Date	2015-10-21
Publisher Institution	The American Society for Mass Spectrometry
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Single-probe mass spectrometry High spatial resolution mass spectrometry imaging Ambient mass spectrometry imaging Liquid surface microextraction Dicationic compounds Paired ion electrospray ionization Analytical Chemistry Biotechnology Organic Chemistry Proteomics Bioinformatics
Content Type	Text
Resource Type	Article
Subject	Spectroscopy Structural Biology

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