NDLI: PCR-RFLP analysis of the ITS2 region to identify Japanese Lymantria species (Lepidoptera: Lymantriidae)

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PCR-RFLP analysis of the ITS2 region to identify Japanese Lymantria species (Lepidoptera: Lymantriidae)

Content Provider	Springer Nature Link
Author	Arimoto, Makoto Iwaizumi, Ren
Copyright Year	2015
Abstract	In this study, a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)-based method for species identification was applied to eight Japanese Lymantria species, including the four “Asian gypsy moth” species [Lymantria dispar japonica (Motschulsky), Lymantria umbrosa (Butler), Lymantria albescens Hori and Umeno, and Lymantria postalba Inoue] and four other species [Lymantria xylina Swinhoe, Lymantria mathura Moore, Lymantria monacha (Linnaeus), and Lymantria lucescens (Butler)]. We sequenced the second internal transcribed spacer (ITS2) region of nuclear ribosomal DNA (rDNA) of one individual from each of the eight species. We analyzed the recognition sites of a restriction endonuclease (MnlI) to identify the Lymantria species by PCR-RFLP. We then applied PCR-RFLP analysis to 330 individuals from 54 populations of the 8 species. As a result, these species could be correctly identified with the exception of two species pairs (L. dispar japonica and L. umbrosa; L. albescens and L. postalba). These results suggest that this PCR-RFLP method based on the ITS2 region is useful for identifying Japanese Lymantria species.
Starting Page	63
Ending Page	70
Page Count	8
File Format	PDF
ISSN	00036862
Journal	Applied Entomology and Zoology
Volume Number	51
Issue Number	1
e-ISSN	1347605X
Language	English
Publisher	Springer Japan
Publisher Date	2015-10-16
Publisher Place	Tokyo
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Asian gypsy moth Lymantria dispar japonica (Motschulsky) Plant quarantine Ribosomal DNA Species identification Entomology Agriculture Applied Ecology Plant Pathology Zoology Environmental Management
Content Type	Text
Resource Type	Article
Subject	Insect Science

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Nocturnal flight activities of the female Asian gypsy moth, Lymantria dispar (Linnaeus) (Lepidoptera: Lymantriidae)

The Braconid (Hymenoptera) Parasites of the Gypsy Moth, Lymantria dispar (Lepidoptera: Lymantriidae)

Mating Strategies and Variable Mate Guarding Behavior of Gypsy Moth, Lymantria dispar japonica L., Males (Lepidoptera; Lymantriidae)

Pre-ovipositional Behaviour of the Female Gypsy Moth, Lymantria dispar L. (Lepidoptera, Lymantriidae)

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The effect of varying alkaloid concentrations on the feeding behavior of gypsy moth larvae, Lymantria dispar (L.) (Lepidoptera: Lymantriidae)

Mixed Infection of the Gypsy Moth, Lymantria dispar japonica MOTSCHULSKY (Lepidoptera : Lymantriidae), in a Larch Forest by Entomophthora aulicae (REICH.) SOROK. and Paecilomyces canadensis (VUILL.) BROWN et SMITH

Group effect in the gypsy moth (Lymantria dispar, Lepidoptera, Lymantriidae) related to the population characteristics and food composition

PCR-RFLP analysis of the ITS2 region to identify Japanese Lymantria species (Lepidoptera: Lymantriidae)

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