NDLI: FISH in analysis of gamma ray-induced micronuclei formation in barley

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Journal of Applied Genetics : Volume 51

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Journal of Applied Genetics : Volume 47

FISH in analysis of gamma ray-induced micronuclei formation in barley

Content Provider	Springer Nature Link
Author	Juchimiuk Kwasniewska, Jolanta Brodziak, Lidia Maluszynska, Jolanta
Copyright Year	2010
Abstract	A micronucleus test in combination with fluorescent in situ hybridization (FISH) using telomere-, centromere-specific probes and 5S and 25S rDNA was used for a detailed analysis of the effects of gamma ray irradiation on the root tip meristem cells of barley, Hordeum vulgare (2n = 14). FISH with four DNA probes was used to examine the involvement of specific chromosomes or chromosome fragments in gamma ray-induced micronuclei formation and then to explain their origin. Additionally, a comparison of the possible origin of the micronuclei induced by physical and chemical treatment: maleic hydrazide (MH) and N-nitroso-N-methylurea (MNU) was done. The micronuclei induced by gamma ray could originate from acentric fragments after chromosome breakage or from whole lagging chromosomes as a result of a dysfunction of the mitotic apparatus. No micronuclei containing only centromeric signals were found. An application of rDNA as probes allowed it to be stated that 5S rDNA–bearing chromosomes are involved in micronuclei formation more often than NOR chromosomes. This work allowed the origin of physically- and chemically-induced micronuclei in barley cells to be compared: the origin of micronuclei was most often from terminal fragments. FISH confirmed its usefulness in the characterization of micronuclei content, as well as in understanding and comparing the mechanisms of the actions of mutagens applied in plant genotoxicity.
Starting Page	23
Ending Page	29
Page Count	7
File Format	PDF
ISSN	12341983
Journal	Journal of Applied Genetics
Volume Number	52
Issue Number	1
e-ISSN	21903883
Language	English
Publisher	Springer-Verlag
Publisher Date	2010-12-07
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Barley FISH Gamma ray Micronuclei Plant Genetics & Genomics Microbial Genetics and Genomics Human Genetics Animal Genetics and Genomics Life Sciences
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine

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