NDLI: Low-dose oral immunization with lyophilized tissue of herbicide-resistant lettuce expressing hepatitis B surface antigen for prototype plant-derived vaccine tablet formulation

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Low-dose oral immunization with lyophilized tissue of herbicide-resistant lettuce expressing hepatitis B surface antigen for prototype plant-derived vaccine tablet formulation

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Journal of Applied Genetics : Volume 52, Issue 1, February 2011

Journal of Applied Genetics : Volume 51

Journal of Applied Genetics : Volume 50

Journal of Applied Genetics : Volume 49

Journal of Applied Genetics : Volume 48

Journal of Applied Genetics : Volume 47

Low-dose oral immunization with lyophilized tissue of herbicide-resistant lettuce expressing hepatitis B surface antigen for prototype plant-derived vaccine tablet formulation

Content Provider	Springer Nature Link
Author	Pniewski, Tomasz Kapusta, Józef Bociąg, Piotr Wojciechowicz, Jacek Kostrzak, Anna Gdula, Michał Fedorowicz Strońska, Olga Wójcik, Piotr Otta, Halina Samardakiewicz, Sławomir Wolko, Bogdan Płucienniczak, Andrzej
Copyright Year	2010
Abstract	Efficient immunization against hepatitis B virus (HBV) and other pathogens with plant-based oral vaccines requires appropriate plant expressors and the optimization of vaccine compositions and administration protocols. Previous immunization studies were mainly based on a combination of the injection of a small surface antigen of HBV (S-HBsAg) and the feeding with raw tissue containing the antigen, supplemented with an adjuvant, and coming from plants conferring resistance to kanamycin. The objective of this study was to develop a prototype oral vaccine formula suitable for human immunization. Herbicide-resistant lettuce was engineered, stably expressing through progeny generation micrograms of S-HBsAg per g of fresh weight and formed into virus-like particles (VLPs). Lyophilized tissue containing a relatively low, 100-ng VLP-assembled antigen dose, administered only orally to mice with a long, 60-day interval between prime and boost immunizations and without exogenous adjuvant, elicited mucosal and systemic humoral anti-HBs responses at the nominally protective level. Lyophilized tissue was converted into tablets, which preserved S-HBsAg content for at least one year of room temperature storage. The results of the study provide indications on immunization methodology using a durable, efficacious, and convenient plant-derived prototype oral vaccine against hepatitis B.
Starting Page	125
Ending Page	136
Page Count	12
File Format	PDF
ISSN	12341983
Journal	Journal of Applied Genetics
Volume Number	52
Issue Number	2
e-ISSN	21903883
Language	English
Publisher	Springer-Verlag
Publisher Date	2010-11-23
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Anti-HBV oral vaccine Lyophilized plant tissue Oral immunization Plant-based vaccines S-HBsAg Transgenic lettuce Animal Genetics and Genomics Microbial Genetics and Genomics Plant Genetics & Genomics Life Sciences Human Genetics
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine

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Low-dose oral immunization with lyophilized tissue of herbicide-resistant lettuce expressing hepatitis B surface antigen for prototype plant-derived vaccine tablet formulation

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