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| Content Provider | Springer Nature Link |
|---|---|
| Author | Megyeri, Mária Mikó, Péter Farkas, András Molnár Láng, Márta Molnár, István |
| Copyright Year | 2016 |
| Abstract | The cytomolecular discrimination of the A$^{m}$- and A-genome chromosomes facilitates the selection of wheat-Triticum monococcum introgression lines. Fluorescence in situ hybridisation (FISH) with the commonly used DNA probes Afa family, 18S rDNA and pSc119.2 showed that the more complex hybridisation pattern obtained in T. monococcum relative to bread wheat made it possible to differentiate the A$^{m}$ and A chromosomes within homoeologous groups 1, 4 and 5. In order to provide additional chromosomal landmarks to discriminate the A$^{m}$ and A chromosomes, the microsatellite repeats (GAA)$_{n}$, (CAG)$_{n}$, (CAC)$_{n}$, (AAC)$_{n}$, (AGG)$_{n}$ and (ACT)$_{n}$ were tested as FISH probes. These showed that T. monococcum chromosomes have fewer, generally weaker, simple sequence repeat (SSR) signals than the A-genome chromosomes of hexaploid wheat. A differential hybridisation pattern was observed on 6A$^{m}$ and 6A chromosomes with all the SSR probes tested except for the (ACT)$_{n}$ probe. The 2A$^{m}$ and 2A chromosomes were differentiated by the signals given by the (GAA)$_{n}$, (CAG)$_{n}$ and (AAC)$_{n}$ repeats, while only (GAA)$_{n}$ discriminated the chromosomes 3A$^{m}$ and 3A. Chromosomes 7A$^{m}$ and 7A could be differentiated by the lack of (GAA)$_{n}$ and (AGG)$_{n}$ signals on 7A. As potential landmarks for identifying the A$^{m}$ chromosomes, SSR repeats will facilitate the introgression of T. monococcum chromatin into wheat. |
| Starting Page | 67 |
| Ending Page | 70 |
| Page Count | 4 |
| File Format | |
| ISSN | 12341983 |
| Journal | Journal of Applied Genetics |
| Volume Number | 58 |
| Issue Number | 1 |
| e-ISSN | 21903883 |
| Language | English |
| Publisher | Springer Berlin Heidelberg |
| Publisher Date | 2016-07-28 |
| Publisher Place | Berlin, Heidelberg |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Triticum monococcum Microsatellite repeats Karyotypic analysis FISH Plant Genetics & Genomics Human Genetics Animal Genetics and Genomics Microbial Genetics and Genomics |
| Content Type | Text |
| Resource Type | Article |
| Subject | Genetics Medicine |
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