NDLI: Self Replicating Gene Vaccine Carrying P1-2A Gene of FMDV Serotype O and its Effects on the Immune Responses of Cattle

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Self Replicating Gene Vaccine Carrying P1-2A Gene of FMDV Serotype O and its Effects on the Immune Responses of Cattle

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VirusDisease : Volume 21

Self Replicating Gene Vaccine Carrying P1-2A Gene of FMDV Serotype O and its Effects on the Immune Responses of Cattle

Content Provider	Springer Nature Link
Author	Nagarajan, G. Ravikumar, P. Ashok Kumar, C. Reddy, G. R. Dechamma, H. J. Suryanarayana, V. V. S.
Copyright Year	2011
Abstract	DNA vaccines are considered as alternatives to live attenuated ones for those diseases like foot-and-mouth disease (FMD) where the production and application of live vaccines have been found unsuccessful. However, stability of DNA and the quantity of antigen expressed are the major limitation with naked DNA vaccines. To address these issues self replicating gene vaccine construct was made for foot-and-mouth disease virus (FMDV) type ‘O’ and studied. The vector for vaccine construct, designated as pSinCMVVac carried CMV promoter and Poly(A) signal sequences at 5′ and 3′ end of Sindbis replicase gene respectively. Gene for structural protein precursor (P1-2A) of FMDV serotype ‘O’ was inserted into pSinCMVVac under subgenomic promoter. 5′UTR (untranslated region) of FMDV was introduced upstream of P1-2A to enhance the level of expression of cloned gene. Functionality of the vaccine construct was confirmed in vitro and in vivo. The self-replicating gene vaccine construct was tested in cattle in comparison with naked DNA vaccine carrying P1-2A and 3CD (pUP3CD). Humoral immune response by ELISA and SNT and cellular response by lymphoproliferation assay using MTT were studied. The default approach of using self replicating gene vaccine in high dose and multiple injection in cattle as followed in our studies might result in immunosuppression as this was observed in our subsequent experiments in guinea pigs. Hence based on dose response studies, vaccine strategy needs to be decided. However, the approach of using Sindbis polymerase gene and UTR in FMDV vaccine is the first report and shows future scope of developing such vaccines.
Starting Page	50
Ending Page	58
Page Count	9
File Format	PDF
ISSN	09702822
Journal	VirusDisease
Volume Number	22
Issue Number	1
e-ISSN	09740120
Language	English
Publisher	Springer-Verlag
Publisher Date	2011-05-10
Publisher Place	India
Access Restriction	Subscribed
Subject Keyword	FMD Self replicating RNA vaccine Sindbis replicase gene Immune response Cattle Protein Structure Life Sciences Biochemistry Cell Biology Microbiology
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Virology

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Self Replicating Gene Vaccine Carrying P1-2A Gene of FMDV Serotype O and its Effects on the Immune Responses of Cattle.

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Self Replicating Gene Vaccine Carrying P1-2A Gene of FMDV Serotype O and its Effects on the Immune Responses of Cattle

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Self Replicating Gene Vaccine Carrying P1-2A Gene of FMDV Serotype O and its Effects on the Immune Responses of Cattle