NDLI: Protective immune response of live attenuated thermo-adapted peste des petits ruminants vaccine in goats

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Protective immune response of live attenuated thermo-adapted peste des petits ruminants vaccine in goats

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Protective immune response of live attenuated thermo-adapted peste des petits ruminants vaccine in goats

Content Provider	Springer Nature Link
Author	Balamurugan, V. Sen, A. Venkatesan, G. Bhanuprakash, V. Singh, R. K.
Copyright Year	2014
Abstract	Virulent isolate of peste des petits ruminants virus (PPRV) of Indian origin (PPRV Jhansi 2003) initially adapted in Vero cells was further propagated in thermo-adapted (Ta) Vero cells grown at 40 °C for attaining thermo-adaption and attenuation of virus for development of Ta vaccine against PPR in goats and sheep. The virus was attenuated up to 50 passages in Ta Vero cells, at which, the virus was found sterile, innocuous in mice and guinea pigs and safe in seronegative goats and sheep. The developed vaccine was tested for its immunogenicity in goats and sheep by subcutaneous inoculation of 100 TCID$_{50}$ (0.1 field dose), 10$^{3}$ TCID$_{50}$ (one field dose) and 10$^{5}$ TCID$_{50}$ (100 field doses) of the attenuated virus along with controls as per OIE described protocols for PPR vaccine testing and were assessed for PPRV-specific antibodies 7–28 days post vaccination (dpv) by PPR competitive ELISA and serum neutralization tests. The PPRV antibodies were detected in all immunized goats and sheep and goats were protective when challenged with virulent PPRV at 28th dpv along with controls for potency testing of the vaccine. The attenuated vaccine did not induce any adverse reaction at high dose (10$^{5}$ TCID$_{50}$) in goats and sheep and provided complete protection even at low dose (10$^{2}$ TCID$_{50}$) in goats when challenged with virulent virus. There was no shedding and horizontal transmission of the attenuated virus to in-contact controls. The results indicate that the developed PPR Ta attenuated virus is innocuous, safe, immunogenic and potent or efficacious vaccine candidate alternative to the existing vaccines for the protection of goats and sheep against PPR in the tropical countries like India.
Starting Page	350
Ending Page	357
Page Count	8
File Format	PDF
ISSN	23473584
Journal	VirusDisease
Volume Number	25
Issue Number	3
e-ISSN	23473517
Language	English
Publisher	Springer India
Publisher Date	2014-03-29
Publisher Place	India
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	PPR virus Thermo-adapted Vaccine Efficacy Goats and sheep Microbiology Life Sciences Biochemistry Protein Structure Cell Biology
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Virology

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Protective immune response of live attenuated thermo-adapted peste des petits ruminants vaccine in goats.

Protective immune response of live attenuated thermo-adapted peste des petits ruminants vaccine in goats

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Protective immune response of live attenuated thermo-adapted peste des petits ruminants vaccine in goats

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