NDLI: Molecular Characterization of Nucleopolyhedrovirus of Three Lepidopteran Pests Using Late Expression Factor-8 Gene

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Abstracts of the Papers (Oral/Poster) Presented During the XXI National Conference of Indian Virological Society (IVS) on “Immunobiology and Management of Viral Diseases in 21st Century”, Held from 8–10 November, 2012, at Indian Veterinary Research Institute (IVRI) Mukteswar Campus, Mukteswar, Nainital 263138, Uttarakhand

VirusDisease : Volume 23

VirusDisease : Volume 22

VirusDisease : Volume 21

Molecular Characterization of Nucleopolyhedrovirus of Three Lepidopteran Pests Using Late Expression Factor-8 Gene

Content Provider	Springer Nature Link
Author	Jose, Jency Jalali, S. K. Shivalingaswamy, T. M. Kumar, N. K. Krishna Bhatnagar, R. Bandyopadhyay, A.
Copyright Year	2013
Abstract	A PCR based method for detection of viral DNA in nucleopolyhedrovirus of three lepidopterans, Spodoptera litura, Amsacta albistriga and Helicoverpa armigera, was developed by employing the late expression factor-8 (lef-8) gene of three NPV using specific primers. The amplicons of 689, 699 and 665 bp were amplified, respectively, and the nucleotide sequences were submitted to GenBank and the accession numbers were obtained. The sequences of lef-8 gene of S. litura NPV and H. armigera NPV matched with those of their respective references in the GenBank database, thereby confirming their identity, however, the sequence of A. albistriga NPV was the first sequence submitted to the GenBank database. The sequence similarity analysis between the three lef-8 gene of NPV sequenced in the present study revealed that there was no significant similarity between them, however A. albistriga NPV and S. litura NPV were found to be closely related. CLUSTAL alignment of the sequences generated revealed general relatedness among NPVs lef-8 gene. The study confirmed that lef-8 gene can be used for quick and correct discriminatory identification of insect viruses.
Starting Page	59
Ending Page	65
Page Count	7
File Format	PDF
ISSN	09702822
Journal	VirusDisease
Volume Number	24
Issue Number	1
e-ISSN	09740120
Language	English
Publisher	Springer-Verlag
Publisher Date	2013-01-29
Publisher Place	India
Access Restriction	Subscribed
Subject Keyword	Nucleopolyhedrovirus Late expression gene Lepidopterans Microbiology Life Sciences Biochemistry Protein Structure Cell Biology
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Virology

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