NDLI: Glycoprotein C Gene Based Molecular Subtyping of a Bovine Herpesvirus -1 Isolate from Uttar Pradesh, India

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Glycoprotein C Gene Based Molecular Subtyping of a Bovine Herpesvirus -1 Isolate from Uttar Pradesh, India

VirusDisease : Volume 23, Issue 2, September 2012

VirusDisease : Volume 23, Issue 1, June 2012

VirusDisease : Volume 22

VirusDisease : Volume 21

Glycoprotein C Gene Based Molecular Subtyping of a Bovine Herpesvirus -1 Isolate from Uttar Pradesh, India

Content Provider	Springer Nature Link
Author	Ravishankar, Chintu Nandi, S. Chander, V. Mohapatra, T. K.
Copyright Year	2012
Abstract	Bovine herpesvirus -1 (BHV-1) is the etiological agent of many clinical syndromes in cattle which causes huge economic losses to the animal husbandry sector annually. Since the first report of its presence in India in 1976, the disease is considered to be endemic in the country. In the present study, a case of keratoconjunctivitis in a cow was investigated to find out the underlying cause of the condition. The clinical material (ocular swab) was tested by BHV-1 glycoprotein D gene specific PCR using in house designed primers and found to be positive by the presence of a 212 bp DNA product in agarose gel electrophoresis. The virus was isolated in MDBK cell line in the third passage and the serum from the animal, was positive for antibodies against BHV-1 by ELISA. A 575 bp segment of the glycoprotein C gene of the isolate was amplified by PCR, cloned and sequenced. On phylogenetic analysis, it was seen that the sequence matched with published BHV-1.1 sequences from USA and Uruguay whereas it was divergent from Brazilian BHV-1.1 isolates. This study highlights the isolation, rapid and sensitive detection of BHV-1 virus from clinical cases and its subtyping by nucleotide sequencing and subsequent phylogenetic analysis which gives invaluable information about the molecular epidemiology of BHV-1 subtypes prevalent in the country.
Starting Page	402
Ending Page	406
Page Count	5
File Format	PDF
ISSN	09702822
Journal	VirusDisease
Volume Number	23
Issue Number	3
e-ISSN	09740120
Language	English
Publisher	Springer-Verlag
Publisher Date	2012-10-12
Publisher Place	India
Access Restriction	Subscribed
Subject Keyword	Bovine herpesvirus 1 Isolation Phylogeny Subtype 1.1 India Biochemistry Life Sciences Protein Structure Microbiology Cell Biology
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Virology

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