NDLI: Comparison of tissue blot immunoassay and reverse transcription polymerase chain reaction assay for virus-testing pulse crops from a South-Eastern Australia survey

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Comparison of tissue blot immunoassay and reverse transcription polymerase chain reaction assay for virus-testing pulse crops from a South-Eastern Australia survey

Content Provider	Springer Nature Link
Author	Freeman, Angela Joan Spackman, Merrin Elizabeth Aftab, Mohammad McQueen, Virginia King, Shane Leur, Joseph A. G. Loh, Mai Hlaing Rodoni, Brendan
Copyright Year	2013
Abstract	A virus survey of field pea (Pisum sativum) and faba bean (Vicia faba) crops in southern NSW and Victoria in October, 2006, provided the opportunity to compare the diagnostic results of a large number of field samples using reverse transcription polymerase chain reaction (RT-PCR) to the accepted reference test of tissue blot immunoassay (TBIA). One hundred unbiased plant samples from each of 21 field pea and three faba bean crops from NSW and ten field pea crops from Victoria were tested for Alfalfa mosaic virus (AMV), Bean yellow mosaic virus (BYMV), Cucumber mosaic virus (CMV), Pea seed-borne mosaic virus (PSbMV), Bean leafroll virus (BLRV) and Beet western yellows virus (BWYV) by TBIA. PSbMV was present at high levels (32–77 %) in 12 out of 21 field pea crops sampled from NSW. BLRV was present at high levels (28–42 %) in faba bean crops from NSW. In Victorian field pea crops, BWYV was most prevalent (4–28 %). Other viruses tested for were present at low incidences or absent. The seed to seedling transmission rate of PSbMV in the field pea seedlots used to sow six of the surveyed NSW pea crops, determined by TBIA, ranged from 0 to 23 % and had a clear effect on the level of PSbMV in the field. Test results for PSbMV and BLRV using TBIA were compared with results using RT-PCR and compared favourably in giving similar estimates of infection incidence and low costs (both less than A$1.00 per sample) for detecting these two viruses. It is concluded that TBIA is the preferred technique to get a precise estimate of infection incidence, while RT-PCR is particularly useful to test bulk samples for the presence of viruses with a low incidence and provides an alternative if antiserum is not available.
Starting Page	675
Ending Page	683
Page Count	9
File Format	PDF
ISSN	08153191
Journal	Australasian Plant Pathology
Volume Number	42
Issue Number	6
e-ISSN	14486032
Language	English
Publisher	Springer Netherlands
Publisher Date	2013-09-28
Publisher Institution	Australasian Plant Pathology Society
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Pulse Virus Survey PCR TBIA Plant Pathology Plant Sciences Agriculture Entomology Ecology
Content Type	Text
Resource Type	Article
Subject	Plant Science

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