NDLI: Analysing diversity in sugarcane resistance gene analogues

Content Provider	Springer Nature Link
Author	Hermann, S. Brumbley, S. McIntyre, C. L.
Copyright Year	2006
Abstract	As resistance genes have been shown to contain conserved motifs and cluster in many plant genomes, the identification of resistance gene analogues can be used as a strategy for both the discovery of DNA markers linked to disease resistance loci and the map-based cloning of disease resistance genes. Sugarcane suffers from many important diseases and an analysis of resistance gene analogues offers a means to identify DNA markers linked to resistance loci. However, sugarcane has the most complex genome of any crop plant and initially it is important to understand the extent of resistance gene analogue diversity in the sugarcane genome before genetic analysis. We review herein how more than 100 expressed sequence tags with homology to different resistance genes have been identified in sugarcane with many mapped as single-dose restriction fragment length polymorphism markers. Importantly, some of these resistance gene analogues have been shown to be linked to disease resistance genes or disease quantitative trait loci. In an attempt to more efficiently analyse additional resistance gene analogues in sugarcane, we report on experiments aimed at investigating the molecular diversity of several resistance gene analogue families using a modified form of a technique termed Ecotilling. Using Ecotilling, we were able to rapidly detect single nucleotide polymorphisms in fragments amplified by PCR from four different resistance gene analogue families, SoRP1D, SoPTO, SoXa21 and SoHs1pro-1. An analysis of a diverse set of sugarcane varieties, including modern sugarcane cultivars and several S. officinarum and S. spontaneum clones, indicated that all amplicons, apart from SoHs1pro-1, contained significant polymorphism within the gene region studied. However, a comparison among these sugarcane clones, including between the parents of two sugarcane mapping populations, indicated that most polymorphisms were multi-dose, not single-dose, preventing their genetic map location or association with disease susceptibility or resistance from being determined.
Starting Page	631
Ending Page	641
Page Count	11
File Format	PDF
ISSN	08153191
Journal	Australasian Plant Pathology
Volume Number	35
Issue Number	6
e-ISSN	14486032
Language	English
Publisher	Springer Netherlands
Publisher Date	2006-01-01
Publisher Institution	Australasian Plant Pathology Society
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Plant Pathology Plant Sciences Agriculture Entomology Ecology
Content Type	Text
Resource Type	Article
Subject	Plant Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Distribution of Sugarcane mosaic virus in sugarcane plants

Sugarcane smut in Queensland: arrival and emergency response

Varietal susceptibility to the corn wireworm Melanotus communis (Coleoptera: Elateridae) in sugarcane

Amendments of sugarcane trash induce suppressiveness to plant-parasitic nematodes in a sugarcane soil

Management of an incursion of sugarcane smut in Australia

Genetic diversity of the nucleocapsid gene of Iris yellow spot virus

A method for rating sugarcane cultivars for resistance to ratoon stunting disease based on an enzyme-linked immunoassay

Disease and diversity in forest ecosystems

Lessons from the tropics — plant diversity, unusual and changeable plant pathology, horizontal resistance, and the plight of farmers

Analysing diversity in sugarcane resistance gene analogues

Similar Documents

Distribution of Sugarcane mosaic virus in sugarcane plants

Sugarcane smut in Queensland: arrival and emergency response

Varietal susceptibility to the corn wireworm Melanotus communis (Coleoptera: Elateridae) in sugarcane

Amendments of sugarcane trash induce suppressiveness to plant-parasitic nematodes in a sugarcane soil

Management of an incursion of sugarcane smut in Australia

Genetic diversity of the nucleocapsid gene of Iris yellow spot virus

A method for rating sugarcane cultivars for resistance to ratoon stunting disease based on an enzyme-linked immunoassay

Disease and diversity in forest ecosystems

Lessons from the tropics — plant diversity, unusual and changeable plant pathology, horizontal resistance, and the plight of farmers

Analysing diversity in sugarcane resistance gene analogues