NDLI: Examining interactions between legumes and Aphanomyces euteiches with real-time PCR

Content Provider	Springer Nature Link
Author	Vandemark, G. J. Ariss, J. J.
Copyright Year	2007
Abstract	Real-time PCR has many applications in the study of host-pathogen interactions including improving the resolution of disease screening programs in selecting highly resistant plants. Identifying the earliest time in the disease process that real-time PCR can be used to reliably identify resistant genotypes will optimise applications of these assays in resistance breeding. Experiments were performed to examine the accumulation of Aphanomyces euteiches in resistant and susceptible pea populations at several different time points (7, 10 and 14 days) during the progress of disease progression. At all times, significantly more A. euteiches DNA was detected in the susceptible cultivar Bolero than in the resistant germplasm 90–2079. The earliest significant correlation between disease severity and pathogen DNA quantitywas observed 14 days after inoculation. In both resistant and susceptible populations, the quantity of A. euteiches DNA reached a maximum at 7 days, and then decreased in a fairly linear manner over time. These observations provide evidence that A. euteiches is a biotrophic organism that primarily produces mycelia early in the disease cycle. These results suggest that control measures be targeted to disrupt the pathogen early in the process of disease to reduce the production of oospores that serve as subsequent field inoculum.
Starting Page	102
Ending Page	108
Page Count	7
File Format	PDF
ISSN	08153191
Journal	Australasian Plant Pathology
Volume Number	36
Issue Number	2
e-ISSN	14486032
Language	English
Publisher	Springer Netherlands
Publisher Date	2007-01-01
Publisher Institution	Australasian Plant Pathology Society
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Plant Pathology Plant Sciences Agriculture Entomology Ecology
Content Type	Text
Resource Type	Article
Subject	Plant Science

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