NDLI: Gazar virus Y, a new member of the celery mosaic virus group of potyviruses, isolated from carrots in Egypt

Content Provider	Springer Nature Link
Author	Soliman, A. M. Ahmed, Amal A. Mohamed, Mervat S. Hanna, Demiana H. Barsoum, B. N.
Copyright Year	2012
Abstract	A novel potyvirus we call Gazar virus Y (GVY) was isolated from diseased carrot plants in Egypt. It produced symptoms similar to those caused by carrot virus Y. Its particles were visualized in leaf extracts using negative stain, and leaf tissues from carrot plants infected with GVY had many ultra-structural differences from healthy ones. Chemical analyses of healthy and infected carrot roots showed that carrot roots infected with GVY had increased dry weight and decreased moisture, total ash, total carbohydrates, total fibres, vitamin C, β-carotene, vitamin A and the minerals of K, Mg, Na, P and Ca compared to the healthy carrot roots. Molecular methods, including reverse transcription-polymerase chain reaction (RT-PCR), were used to amplify a 335 bp cDNA fragment transcribed from infected plant extracts using degenerate oligonucleotide primers specific for potyviruses. The amplified cDNA was cloned into pGEM®-T Easy vector, and transformed into Escherichia coli (E. coli) strain DH5α . The recombinant inserts were sequenced. The nucleotide sequence (GenBank Accession Code GQ148776) was matched against viral nucleotide sequences in the GenBank database. It is most closely related to viruses of the celery mosaic virus group of potyviruses, but is distinct from all and represents a sister lineage to all others described so far.
Starting Page	529
Ending Page	534
Page Count	6
File Format	PDF
ISSN	08153191
Journal	Australasian Plant Pathology
Volume Number	41
Issue Number	5
e-ISSN	14486032
Language	English
Publisher	Springer Netherlands
Publisher Date	2012-05-16
Publisher Institution	Australasian Plant Pathology Society
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Gazar virus Y Celery mosaic virus group Ultra-structural Chemical analyses RT-PCR Agriculture Plant Sciences Ecology Plant Pathology Entomology
Content Type	Text
Resource Type	Article
Subject	Plant Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Incidence of Celery mosaic virus in celery crops in south-west Australia and its management using a ‘celery-free period’

Tobacco mosaic virus RNA as an internal control for duplex RT-PCR assay of pea germplasm

The truth about Pea mild mosaic virus

A survey of viruses infecting Rosa spp. in New Zealand

Improved PCR detection of potyviruses in Allium species

Celery mosaic potyvirus occurring naturally in cultivated Apiaceae in Victoria, Australia

Abacá mosaic virus: A distinct strain of Sugarcane mosaic virus

Multiplex RT-PCR for parallel detection of three RNA viruses associated with stripe and mosaic diseases of sorghum in India

Occurrence of Banana bract mosaic virus on cardamom

Gazar virus Y, a new member of the celery mosaic virus group of potyviruses, isolated from carrots in Egypt

Similar Documents

Incidence of Celery mosaic virus in celery crops in south-west Australia and its management using a ‘celery-free period’

Tobacco mosaic virus RNA as an internal control for duplex RT-PCR assay of pea germplasm

The truth about Pea mild mosaic virus

A survey of viruses infecting Rosa spp. in New Zealand

Improved PCR detection of potyviruses in Allium species

Celery mosaic potyvirus occurring naturally in cultivated Apiaceae in Victoria, Australia

Abacá mosaic virus: A distinct strain of Sugarcane mosaic virus

Multiplex RT-PCR for parallel detection of three RNA viruses associated with stripe and mosaic diseases of sorghum in India

Occurrence of Banana bract mosaic virus on cardamom

Gazar virus Y, a new member of the celery mosaic virus group of potyviruses, isolated from carrots in Egypt