NDLI: An experimental approach for identifying microorganisms involved in specified functions: utilisation for understanding a nematode suppressive soil

Content Provider	Springer Nature Link
Author	Borneman, James Olatinwo, Rabiu Yin, Bei Beckers, J. Ole
Copyright Year	2004
Abstract	An experimental app roach was developed for identifyin g microorganisms involved in spec if ied fun ctions such as path ogen suppress iveness. In thi s approac h, it was postul ated th at the micro organisms involved in path ogen suppress iveness co uld be discovered by identifyin g those orga nisms whose popul ation s positively co rrelate with hi gh levels of suppress iveness. The approac h has thre e phase s. The fir st phase is to identify bacteri al and fungal rRNA genes (rDNA) from so ils possessin g various levels of suppress iveness. Rib osomal DNA sequences that are more abundant in the highly suppress ive so ils than in the less suppress ive soils are considered cand idate sequences. A meth od termed oligonucleotide fin gerprinting of rRNA ge nes (OFRG) is used to obta in extensive analysis of microbi al community composition. The seco nd phase of thi s experimental approac h is to verify the results obtained from phase one usin g quantitative PCR. Here, select ive PCR primers for each of the candidate rDNA sequences are design ed. The se primers are then used to determine the rel ative amounts of the candidate sequences in soils possessin g various levels of suppress iveness produ ced by several different meth ods such as mixing various quantitie s of suppress ive and fumi gation-induced non-suppressive so il, biocid al treatments and temperature tre atments. In phase three, the organisms th at cons istently correlate with suppress iveness are isolated and amended to non-suppressive soils to ass ess their abilit ies to produ ce suppress iveness. The utility of thi s experimental appro ach was dem onstrated by usin g it to identify microorganisms invo lved in suppress iveness again st the plant-p arasitic nematode, Heterodera schachtii. This general experimental approac h sho uld also be useful for the identifying microorganisms involved in fun ctions other then pathogen suppress iveness.
Starting Page	151
Ending Page	155
Page Count	5
File Format	PDF
ISSN	01560972
Journal	Australasian Plant Pathology
Volume Number	33
Issue Number	2
e-ISSN	14486032
Language	English
Publisher	Springer Netherlands
Publisher Date	2004-01-01
Publisher Institution	Australasian Plant Pathology Society
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Plant Pathology Plant Sciences Agriculture Entomology Ecology
Content Type	Text
Resource Type	Article
Subject	Plant Science

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