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| Content Provider | Springer Nature Link |
|---|---|
| Author | Rodda, M. S. Kant, P. Lindbeck, K. D. Gnanasambandam, A. Hollaway, G. J. |
| Copyright Year | 2015 |
| Abstract | A high-throughput and reliable method to screen field pea germplasm for bacterial blight resistance was developed. The method uses spray inoculation of seedlings in small pots with a bacterial suspension followed by incubation in a glasshouse. This is less laborious compared to previous stab-inoculation methods, and takes less than 1 month (c.a. 25–27 days) from seed sowing to disease assessment. It uses a surfactant to achieve good coverage of bacterial suspension and required leaf wetness for progression of the disease. Disease symptoms in the glasshouse were similar to those observed in field, and data were highly correlated with those from field experiments. The method was validated using individual and combination of isolates of Pseudomonas syringae pathovars pisi and/or syringae to test their ability to effectively differentiate between resistant and susceptible pea genotypes. Screening of diverse field pea landraces and wild relatives using present screening method has identified potentially new sources of resistance to P. syringae pvs pisi and syringae. The developed method is being used to evaluate bacterial blight resistance within the Australian field pea breeding program. |
| Starting Page | 515 |
| Ending Page | 526 |
| Page Count | 12 |
| File Format | |
| ISSN | 08153191 |
| Journal | Australasian Plant Pathology |
| Volume Number | 44 |
| Issue Number | 5 |
| e-ISSN | 14486032 |
| Language | English |
| Publisher | Springer Netherlands |
| Publisher Date | 2015-09-04 |
| Publisher Institution | Australasian Plant Pathology Society |
| Publisher Place | Dordrecht |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Disease screening Pathovars Inoculation method Pulse® penetrant Resistance Pseudomonas syringae Plant Pathology Plant Sciences Agriculture Entomology Ecology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Plant Science |
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